Anti-Somatostatin抗体(ab108456)
Key features and details
- Rabbit polyclonal to Somatostatin
- Suitable for: IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Somatostatin抗体
参阅全部 Somatostatin 一抗 -
描述
兔多克隆抗体to Somatostatin -
宿主
Rabbit -
经测试应用
适用于: IHC-Pmore details
不适用于: WB -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Sheep, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Gorilla, Orangutan -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- IHC-P: Rat, Mouse and Human Pancreas tissue
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab108456于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P | (3) |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Somatostatin inhibits the release of somatotropin. -
序列相似性
Belongs to the somatostatin family. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 280932 Cow
- Entrez Gene: 403993 Dog
- Entrez Gene: 6750 Human
- Entrez Gene: 20604 Mouse
- Entrez Gene: 494469 Pig
- Entrez Gene: 24797 Rat
- Entrez Gene: 443006 Sheep
- Omim: 182450 Human
see all -
别名
- Growth hormone release inhibiting factor antibody
- Growth hormone release-inhibiting factor antibody
- Prepro somatostatin antibody
see all
图片
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IHC image of Somatostatin staining in a section of formalin-fixed paraffin-embedded normal rat pancreas performed on a Leica BOND™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab108456, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
IHC image of Somatostatin staining in a section of formalin-fixed paraffin-embedded normal mouse pancreas performed on a Leica BOND™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab108456, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
IHC image of Somatostatin staining in Human normal pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab108456, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
数据表及文件
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SDS download
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Datasheet download
文献 (9)
ab108456 被引用在 9 文献中.
- Nestor-Kalinoski A et al. Unique Neural Circuit Connectivity of Mouse Proximal, Middle, and Distal Colon Defines Regional Colonic Motor Patterns. Cell Mol Gastroenterol Hepatol 13:309-337.e3 (2022). PubMed: 34509687
- Huang R et al. Epidural electrical stimulation of the cervical spinal cord opposes opioid-induced respiratory depression. J Physiol 600:2973-2999 (2022). PubMed: 35639046
- Carter AM et al. Cdk5 drives formation of heterogeneous pancreatic neuroendocrine tumors. Oncogenesis 10:83 (2021). PubMed: 34862365
- Singh B et al. KATP channel blockers control glucagon secretion by distinct mechanisms: A direct stimulation of α-cells involving a [Ca2+]c rise and an indirect inhibition mediated by somatostatin. Mol Metab 53:101268 (2021). PubMed: 34118477
- Murayama AY et al. The polymicrogyria-associated GPR56 promoter preferentially drives gene expression in developing GABAergic neurons in common marmosets. Sci Rep 10:21516 (2020). PubMed: 33299078
- Waller R et al. Histological characterization of interneurons in Alzheimer's disease reveals a loss of somatostatin interneurons in the temporal cortex. Neuropathology 40:336-346 (2020). PubMed: 32232904
- Stilwell JM et al. Retrobulbar gangliocytoma and carcinoma of the corpuscles of Stannius in a pearl gourami, Trichopodus leerii (Bleeker, 1852). J Fish Dis 43:1473-1478 (2020). PubMed: 32851692
- Espinosa N et al. Basal Forebrain Gating by Somatostatin Neurons Drives Prefrontal Cortical Activity. Cereb Cortex 29:42-53 (2019). PubMed: 29161383
- Alfonsi G & Crippa M Updates on the Methodological Approaches for Carrying Out an In-Depth Study of the Cardiac Conduction System and the Autonomic Nervous System of Victims of Sudden Unexplained Fetal and Infant Death. Front Med (Lausanne) 3:54 (2016). PubMed: 27917382