重组
RabMAb

Anti-Somatostatin 28抗体[EPR3359(2)] (ab111912)

概述

  • 产品名称
    Anti-Somatostatin 28抗体[EPR3359(2)]
    参阅全部 Somatostatin 28 一抗
  • 描述
    兔单克隆抗体[EPR3359(2)] to Somatostatin 28
  • 经测试应用
    适用于: IHC-P, IHC-Frmore details
    不适用于: Flow Cyt,ICC,IP or WB
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide, corresponding to residues in Human Somatostatin 28.

  • 阳性对照
    • Human pancreas tissue
  • 常规说明

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab111912 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.
  • 应用说明
    Is unsuitable for Flow Cyt,ICC,IP or WB.
  • 靶标

    Anti-Somatostatin 28 antibody [EPR3359(2)] 图像

    • ab111912, at a 1/50 dilution, staining Somatostatin 28 in paraffin embedded Human pancreas tissue by Immunohistochemistry.
    • Ab111912 staining Somatostatin-28 in Rat hippocampus tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 4% paraformaldehyde and permeabilized with 0.2% TritonX-100. Heat mediated antigen retrieval was performed using a citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20).  Primary antibody was used at a 1:500 dilution. Ab150077 (1:1000) was used as the secondary antibody. Counterstained with DAPI. Cytoplasmic staining in the hilus of the dentate gyrus of rat hippocampus [PMID: 25469233] . 

    • ab111912 staining Somatostatin 28 in Mouse pancreas tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Heat mediated antigen retrieval in a TRIS/EDTA buffer pH9 (ab93684). Primary antibody at 1:4000 dilution and   a  Goat Anti- rabbit IgG H&L (HRP) was used as the secondary antibody. Couterstained with hematoxylin. Cytoplasmic staining on delta cells of mouse pancreas islet [PMID: 25019573], [PMID: 9066918], [BiologiaSerbica 2014, Vol. 36  No. 1-2 24-32] . 

       

       

    • Ab111912 staining Somatostatin-28 in Rat pancreas tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 4% paraformaldehyde and permeabilized with 0.2% TritonX-100. Heat mediated antigen retrieval was performed using a citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20).Primary antibody was used at a 1:500 dilution. Ab150077 (1:1000) was used as the secondary antibody.Counterstained with DAPI. Cytoplasmic staining on delta cells of rat pancreas islet. [PMID: 25019573], [PMID: 9066918], [BiologiaSerbica 2014, Vol. 36  No. 1-2 24-32] .  

    • Ab111912 staining Somatostatin-28 in Mouse hippocampus tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 4% paraformaldehyde and permeabilized with 0.2% TritonX-100.Heat mediated antigen retrieval was performed using a citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20). Primary antibody was used at a 1:500 dilution. Ab150077 (1:1000) was used as the secondary antibody.Counterstained with DAPI.  Cytoplasmic staining in the hilus of the dentate gyrus of mouse hippocampus [PMID: 25469233]. 

    • Ab111912 staining Somatostatin-28 in Mouse pancreas tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 4% paraformaldehyde and permeabilized with 0.2% TritonX-100. Heat mediated antigen retrieval was performed using a citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20). Primary antibody was used at a 1:500 dilution. Ab150077 (1:1000) was used as the secondary antibody. Counterstained with DAPI. Cytoplasmic staining on delta cells of mouse pancreas islet. [PMID: 25019573], [PMID: 9066918], [BiologiaSerbica 2014, Vol. 36  No. 1-2 24-32] . 

    • ab111912 staining Somatostatin 28 in Rat pancreas tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Antigen retrieval was by heat mediated in a TRIS/EDTA buffer pH9 (ab93684).Primary antibody at 1:4000 dilution and Goat Anti- rabbit IgG H&L (HRP) was used as the secondary antibody. Counterstained with Hematoxylin. Cytoplasmic staining on delta cells of rat pancreas islet[PMID: 25019573], [PMID: 9066918], [BiologiaSerbica 2014, Vol. 36  No. 1-2 24-32]  .

       

       

    • ab111912 staining Somatostatin 28 in human pancreas tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Heat mediated antigen retrieval in a TRIS/EDTA buffer pH9 (ab93684). Primary antibody  at 1/4000 dilution and a Goat Anti- rabbit IgG H&L (HRP) was used as the secondary antibody. Counterstained with Hematoxylin. Cytoplasmic staining on delta cells of human pancreas islet. [PMID: 25019573], [PMID: 9066918],  [BiologiaSerbica 2014, Vol. 36  No. 1-2 24-32] . 

    Anti-Somatostatin 28 antibody [EPR3359(2)] (ab111912)参考文献

    This product has been referenced in:
    • Simonet JC  et al. Conditional Loss of Arx From the Developing Dorsal Telencephalon Results in Behavioral Phenotypes Resembling Mild Human ARX Mutations. Cereb Cortex N/A:N/A (2014). Mouse . Read more (PubMed: 24794919) »

    See 1 Publication for this product

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