Anti-Soluble TNF Receptor II抗体(ab10502)


  • 产品名称Anti-Soluble TNF Receptor II抗体
    参阅全部 Soluble TNF Receptor II 一抗
  • 描述
    山羊多克隆抗体to Soluble TNF Receptor II
  • 特异性By ELISA, the antibody shows no cross-reactivity with recombinant human sTNF-RI. In addition, the antibody shows no cross-reactivity with other cytokines tested: rhTGF-beta1, pTGF-beta1.2, pTGF-beta2, rcTGF-beta3, raTGF-beta5, rhLatent TGF-beta1, rhTGF-alpha, rhEGF, rhPDGF-AA, rhPDGF-BB, rhPDGF-AB, rhFGF acidic, rhFGF basic, rhFGF-4, rhIL-1a, rmIL-1a, rhIL-1ra, rhIL-1b, rmIL-1b, rhIL-2, rhIL-3, rmIL-3, rhIL-4, rmIL-4, rhIL-5, rmIL-5, rhIL-6, rmIL-6, rhIL-7, rmIL-7, rhIL-8, rhIL-9, rmIL-9, rhIL-10, rhIL-11, rhGM-CSF, rhM-CSF, rhLIF, rhIL-6 sR, rhMIP-1alpha, rmMIP-1alpha, rhMIP-1beta, rmMIP-1beta, rhTNF-alpha, rmTNF-alpha, rhTNF-beta, rhRANTES, rhANG, rhOSM, rhSCF, rmSCF, rhSLPI, rhCNTF, rhIFN-gamma, rhIGF-I, rhIGF-II, rhGRO, rhEpo, rhMCP-1.
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant human TNF soluble receptor II (TNF sRII), expressed in E. coli. This recombinant protein represents the non-glycosylated,N-terminal methionyl form of the naturally occuring human soluble type II receptor for TNF, minus 53 amino acids from the proline-rich region just exterior to the transmembrane domain.

  • 常规说明Endotoxin level is < 10 ng per mg antibody as determined by the LAL method.



Our Abpromise guarantee covers the use of ab10502 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
  • 应用说明ELISA: Use at a concentration of 0.5 - 1 µg/ml, this is determined to detect recombinant human sTNF RII to a limit of 78 pg/well.
    WB: Use at a concentration of 1 - 2 µg/ml, this is determined using recombinant human sTNF RII at 5 ng/lane under non-reducing conditions. Predicted molecular weight: 75 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.
    • 序列相似性Contains 4 TNFR-Cys repeats.
    • 翻译后修饰Phosphorylated; mainly on serine residues and with a very low level on threonine residues.
      A soluble form (tumor necrosis factor binding protein 2) is produced from the membrane form by proteolytic processing.
    • 细胞定位Secreted and Cell membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CD120b antibody
      • p75 antibody
      • p75 TNF receptor antibody
      • p75TNFR antibody
      • p80 TNF alpha receptor antibody
      • p80 TNF-alpha receptor antibody
      • Soluble TNFR1B variant 1 antibody
      • TBP-2 antibody
      • TBPII antibody
      • TNF R II antibody
      • TNF R2 antibody
      • TNF R75 antibody
      • TNF-R2 antibody
      • TNF-RII antibody
      • TNFBR antibody
      • TNFR-II antibody
      • TNFR1B antibody
      • TNFR2 antibody
      • TNFR80 antibody
      • Tnfrsf1b antibody
      • TNR1B_HUMAN antibody
      • Tumor necrosis factor beta receptor antibody
      • Tumor necrosis factor binding protein 2 antibody
      • Tumor necrosis factor receptor 2 antibody
      • Tumor necrosis factor receptor superfamily, member 1B antibody
      • Tumor necrosis factor receptor type II antibody
      • Tumor necrosis factor-binding protein 2 antibody
      see all

    Anti-Soluble TNF Receptor II antibody (ab10502)参考文献

    ab10502 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (Hepatoma cells)
    Loading amount 10 µg
    Specification Hepatoma cells
    Treatment 2 ug TNFR2 for 24h
    Gel Running Conditions Reduced Denaturing (12%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Ms. Hyejeong Shin

    Verified customer

    提交于 Jul 09 2010

    Thank you for your enquiry. For both products, the immunogen is the non-glycosylated, N-terminal methionyl form of the naturally-occurring human soluble type II receptor for TNF, minus 53 amino acids from the proline-rich region just exterior to the...

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