Anti-Soluble TNF Receptor I抗体(ab9979)
Key features and details
- Rabbit polyclonal to Soluble TNF Receptor I
- Suitable for: ELISA, Neutralising, Sandwich ELISA, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Soluble TNF Receptor I抗体
参阅全部 Soluble TNF Receptor I 一抗 -
描述
兔多克隆抗体to Soluble TNF Receptor I -
宿主
Rabbit -
经测试应用
适用于: ELISA, Neutralising, Sandwich ELISA, WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein corresponding to Human Soluble TNF Receptor I aa 1-250.
Database link: P19438 -
阳性对照
- Recombinant human Soluble TNF Receptor I protein (ab9980) can be used as a positive control in WB.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Lyophilized:Reconstitute with 200µl of sterile water. -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
No preservative, sterile filtered -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
轻链类型
unknown -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
应用 | Ab评论 | 说明 |
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ELISA |
Use at an assay dependent concentration.
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Neutralising |
Use at an assay dependent concentration.
To yield one-half maximal inhibition [ND50] of the biological activity of hsTNF-R1 (0.3 µg/ml), a concentration of 0.9 - 1.1 µg/ml of this antibody is required. |
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Sandwich ELISA |
Use at an assay dependent concentration.
To detect hsTNF-receptor by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ab271211 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hsTNF-receptor. |
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WB |
Use at an assay dependent concentration.
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说明 |
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ELISA
Use at an assay dependent concentration. |
Neutralising
Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of hsTNF-R1 (0.3 µg/ml), a concentration of 0.9 - 1.1 µg/ml of this antibody is required. |
Sandwich ELISA
Use at an assay dependent concentration. To detect hsTNF-receptor by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ab271211 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hsTNF-receptor. |
WB
Use at an assay dependent concentration. |
靶标
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相关性
TNF RI (CD120a) is a 55 kDa transmembrane glycoprotein that is expressed by virtually all nucleated mammalian cells. Among the numerous cells known to express TNF RI are hepatocytes, monocytes and neutrophils, cardiac muscle cells, endothelial cells, and CD34+ hematopoietic progenitors. Both TNF alpha and TNF beta bind to TNF RI. Soluble TNF alpha binds with a Kda in the range of 20-60 pM, while TNF beta binds with a Kda equal to 650 pM. TNF RI relative to TNF RII seems to be the more physiologically relevant receptor, whereas TNF R2 appears to play a direct role in only a limited number of TNF responses. Soluble TNF RI, which blocks TNF alpha activity, has been identified in both urine and blood (1-3 ng/mL). Serum levels of sTNF receptors increase dramatically in certain pathological situations. -
细胞定位
Cytoplasmic -
数据库链接
- Entrez Gene: 7132 Human
- Omim: 191190 Human
- SwissProt: P19438 Human
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别名
- CD120a antibody
- TBP1 antibody
- TNFR1 antibody
see all
数据表及文件
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Datasheet download
文献 (0)
ab9979 尚未被引用在任何文献中。