Anti-Soluble Liver/Pancreas Antigen抗体(ab56033)

概述

  • 产品名称
    Anti-Soluble Liver/Pancreas Antigen抗体
  • 描述
    兔多克隆抗体to Soluble Liver/Pancreas Antigen
  • 特异性
    This antibody reacts with Soluble Liver/Pancreas Antigen isoform 2, NP_722547.
  • 经测试应用
    适用于: WB, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide (Human)

  • 阳性对照
    • Jurkat cell lysate (Western Blot). Human Kidney tissue (IHC-P).

性能

应用

Our Abpromise guarantee covers the use of ab56033 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 49 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

靶标

  • 功能
    Converts O-phosphoseryl-tRNA(Sec) to selenocysteinyl-tRNA(Sec) required for selenoprotein biosynthesis.
  • 组织特异性
    Primarily expressed in liver, pancreas, kidney and lung. Overexpressed in PHA-stimulated T-cells.
  • 通路
    Aminoacyl-tRNA biosynthesis; selenocysteinyl-tRNA(Sec) biosynthesis; selenocysteinyl-tRNA(Sec) from L-seryl-tRNA(Sec) (archaeal/eukaryal route): step 2/2.
  • 疾病相关
    Defects in SEPSECS are the cause of pontocerebellar hypoplasia type 2D (PCH2D) [MIM:613811]. PCH2D is a disorder characterized by postnatal onset of progressive atrophy of the cerebrum and cerebellum, microcephaly, profound mental retardation, spasticity, and variable seizures.
  • 序列相似性
    Belongs to the SepSecS family.
  • 细胞定位
    Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZp434B1417 antibody
    • Liver pancreas antigen antibody
    • Liver-pancreas antigen antibody
    • LP antibody
    • MGC161491 antibody
    • O phosphoseryl tRNA(Sec) selenium transferase antibody
    • O-phosphoseryl-tRNA(Sec) selenium transferase antibody
    • Sec synthase antibody
    • Selenocysteine synthase antibody
    • Selenocysteinyl tRNA(Sec) synthase antibody
    • Selenocysteinyl-tRNA(Sec) synthase antibody
    • Sep (O phosphoserine) tRNA:Sec (selenocysteine) tRNA synthase antibody
    • Sep tRNA:Sec tRNA synthase antibody
    • Sep-tRNA:Sec-tRNA synthase antibody
    • SepSecS antibody
    • SLA antibody
    • SLA p35 antibody
    • SLA-p35 antibody
    • SLA/LP antibody
    • SLA/LP autoantigen antibody
    • Soluble liver antigen antibody
    • Soluble liver antigen/liver pancreas antigen antibody
    • SPCS_HUMAN antibody
    • tRNA(Ser/Sec) associated antigenic protein antibody
    • tRNA(Ser/Sec)-associated antigenic protein antibody
    • TRNP48 antibody
    • UGA suppressor tRNA associated protein antibody
    • UGA suppressor tRNA-associated protein antibody
    see all

图片

  • Anti-Soluble Liver/Pancreas Antigen antibody (ab56033) at 1 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 49 kDa
    Observed band size : 54 kDa (why is the actual band size different from the predicted?)
    Gel concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Soluble Liver/Pancreas Antigen with ab56033 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.

文献

ab56033 has not yet been referenced specifically in any publications.

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