Spermatogenesis- and oogenesis-specific basic helix-loop-helix-containing protein 1 antibody
Western blot - Sohlh1 antibody (ab41520)
Anti-Sohlh1 antibody (ab41520) at 2 µg/ml + Mouse Fetus (14 Day Old) Tissue Lysate at 20 µg
Secondary Goat polyclonal to rabbit IgG - H&L - Pre Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 38 kDa Observed band size: 38 kDa Additional bands at: 130 kDa, 73 kDa, 85 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry/ Immunofluorescence - Anti-Sohlh1 antibody (ab41520)Image courtesy of an anonymous Abreview.
ab41520 staining Sohlh1 in in vitro cultures of murine spermatogonia by Immunocytochemistry/ Immunofluorescence. Cells were fixed in paraformaldehyde, permeabilized using 0.1% Triton X-100, blocked with a blocking agent for 1 hour at 25°C and then incubated with ab41520 at a 1/50 dilution for 20 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated chicken anti-rabbit polyclonal, used at a 1/500 dilution.