纯化说明The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against
non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 113 kDa (predicted molecular weight: 113 kDa).
功能This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
序列相似性Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.
翻译后修饰Phosphorylation on Tyr-10 modulates pumping activity.
细胞定位Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.