Anti-Sodium / Hydrogen Exchanger 3 (phospho S552)抗体[14D5] (ab72729)

概述

  • 产品名称
    Anti-Sodium / Hydrogen Exchanger 3 (phospho S552)抗体[14D5]
    参阅全部 Sodium / Hydrogen Exchanger 3 一抗
  • 描述
    小鼠单克隆抗体[14D5] to Sodium / Hydrogen Exchanger 3 (phospho S552)
  • 经测试应用
    适用于: WB, IP, ELISA, ICC/IF, IHC-P, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Rat, Rabbit, Human, Opossum
  • 免疫原

    Synthetic phospho-peptide(rat) made from residues surrounding Serine 552.

  • 阳性对照
    • human kidney lysate
  • 常规说明

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.05% Sodium Azide
    Constituents: Tris glycine, 150mM Sodium chloride
  • Concentration information loading...
  • 纯度
    Protein G purified
  • 克隆
    单克隆
  • 克隆编号
    14D5
  • 同种型
    IgG2a
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab72729 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 93 kDa).
IP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
ICC/IF 1/50 - 1/1000.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

靶标

  • 功能
    Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction.
  • 序列相似性
    Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.
  • 翻译后修饰
    Phosphorylated by PKA, which inhibits activity.
  • 细胞定位
    Membrane. In intestinal epithelial cells, localizes to the ileal brush border.
  • Information by UniProt
  • 数据库链接
  • 别名
    • MGC126718 antibody
    • MGC126720 antibody
    • Na(+)/H(+) exchanger 3 antibody
    • NHE 3 antibody
    • NHE-3 antibody
    • NHE3 antibody
    • SL9A3_HUMAN antibody
    • SLC9A 3 antibody
    • Slc9a3 antibody
    • Sodium/hydrogen exchanger 3 antibody
    • Sodium/hydrogen exchanger, apical epithelial antibody
    • Solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 antibody
    • Solute carrier family 9 (sodium/hydrogen exchanger), member 3 antibody
    • Solute carrier family 9 member 3 antibody
    see all

图片

  • Anti-Sodium / Hydrogen Exchanger 3 (phospho S552) antibody [14D5] (ab72729) at 5 µg/ml + human kidney lysate

    Predicted band size : 93 kDa
    Observed band size : 85 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.

文献

ab72729 has not yet been referenced specifically in any publications.

客户评价及客户问答

Thank you for your enquiry.

The antibody has been tested in Western Blot using Human, Mouse, and Rat Colon, Small Intestine, and Kidney samples. There were bands expressed around 25kDa and 50kDa, with weaker bands at approx. 80-90 kDa observ...

Read More

Thank you for your enquiry. These antibodies are phosphospecific and will not cross react with the non-phospho antigen. They will only detect the whole protein if it is phosphorylated at the repective serine residues. I hope this information hel...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册