Anti-Sodium / Hydrogen Exchanger 3 (phospho S552)抗体[14D5] (ab72729)

概述

  • 产品名称Anti-Sodium / Hydrogen Exchanger 3 (phospho S552)抗体[14D5]
    参阅全部 Sodium / Hydrogen Exchanger 3 一抗
  • 描述
    小鼠单克隆抗体[14D5] to Sodium / Hydrogen Exchanger 3 (phospho S552)
  • 经测试应用适用于: WB, IP, ELISA, ICC/IF, IHC-P, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Rat, Rabbit, Human, Opossum
  • 免疫原

    Synthetic phospho-peptide(rat) made from residues surrounding Serine 552.

  • 阳性对照
    • human kidney lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.05% Sodium Azide
    Constituents: Tris glycine, 150mM Sodium chloride
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆单克隆
  • 克隆编号14D5
  • 同种型IgG2a
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab72729 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 93 kDa).
IP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
ICC/IF 1/50 - 1/1000.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

靶标

  • 功能Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction.
  • 序列相似性Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.
  • 翻译后修饰Phosphorylated by PKA, which inhibits activity.
  • 细胞定位Membrane. In intestinal epithelial cells, localizes to the ileal brush border.
  • Information by UniProt
  • 数据库链接
  • 别名
    • MGC126718 antibody
    • MGC126720 antibody
    • Na(+)/H(+) exchanger 3 antibody
    • NHE 3 antibody
    • NHE-3 antibody
    • NHE3 antibody
    • SL9A3_HUMAN antibody
    • SLC9A 3 antibody
    • Slc9a3 antibody
    • Sodium/hydrogen exchanger 3 antibody
    • Sodium/hydrogen exchanger, apical epithelial antibody
    • Solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 antibody
    • Solute carrier family 9 (sodium/hydrogen exchanger), member 3 antibody
    • Solute carrier family 9 member 3 antibody
    see all

Anti-Sodium / Hydrogen Exchanger 3 (phospho S552) antibody [14D5] 图像

  • Anti-Sodium / Hydrogen Exchanger 3 (phospho S552) antibody [14D5] (ab72729) at 5 µg/ml + human kidney lysate

    Predicted band size : 93 kDa
    Observed band size : 85 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.

Anti-Sodium / Hydrogen Exchanger 3 (phospho S552) antibody [14D5] (ab72729)参考文献

ab72729 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry.

The antibody has been tested in Western Blot using Human, Mouse, and Rat Colon, Small Intestine, and Kidney samples. There were bands expressed around 25kDa and 50kDa, with weaker bands at approx. 80-90 kDa observ...

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Thank you for your enquiry. These antibodies are phosphospecific and will not cross react with the non-phospho antigen. They will only detect the whole protein if it is phosphorylated at the repective serine residues. I hope this information hel...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"