Anti-Sodium/Hydrogen Exchanger 3/NHE-3抗体(ab95299)
Key features and details
- Rabbit polyclonal to Sodium/Hydrogen Exchanger 3/NHE-3
- Suitable for: WB, IHC-P
- Reacts with: Rat
- Isotype: IgG
概述
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产品名称
Anti-Sodium/Hydrogen Exchanger 3/NHE-3抗体
参阅全部 Sodium/Hydrogen Exchanger 3/NHE-3 一抗 -
描述
兔多克隆抗体to Sodium/Hydrogen Exchanger 3/NHE-3 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Rat -
免疫原
Synthetic peptide corresponding to Rat Sodium/Hydrogen Exchanger 3/NHE-3 aa 809-831 (C terminal).
Sequence:DSFLQADGPEEQLQPASPESTHM
Database link: NP_036786 -
阳性对照
- Rat kidney tissue lysate. IHC-P: rat colon tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab95299于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000. Detects a band of approximately 84 kDa (predicted molecular weight: 93 kDa).
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IHC-P |
1/25.
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说明 |
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WB
1/1000. Detects a band of approximately 84 kDa (predicted molecular weight: 93 kDa). |
IHC-P
1/25. |
靶标
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功能
Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction. -
序列相似性
Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family. -
翻译后修饰
Phosphorylated by PKA, which inhibits activity. -
细胞定位
Membrane. In intestinal epithelial cells, localizes to the ileal brush border. - Information by UniProt
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数据库链接
- Entrez Gene: 24784 Rat
- SwissProt: P26433 Rat
- Unigene: 9706 Rat
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别名
- MGC126718 antibody
- MGC126720 antibody
- Na(+)/H(+) exchanger 3 antibody
see all
图片
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Immunohistochemical analysis of paraffin-embedded formalin fixed (4%) rat colon tissue labelling Exchanger 3/NHE-3 with ab95299 at 1/25 dilution, followed by secondary antibody Goat Anti-Rabbit IgG (Alexa Fluor 488) (Green). Membrane and secretory staining on rat colon tissue is observed. Counter stained with Actin-binding Phalloidin-Alexa Fluor 633; DAPI (blue) nuclear stain. (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 (Red) F-Actin stain. (C) Exchanger 3/NHE-3 Antibody (D) Composite image.
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Western blot detection of Sodium/Hydrogen Exchanger 3 / NHE-3 in Rat kidney tissues using ab95299 at 1:1000 dilution.
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab95299 被引用在 5 文献中.
- Zarei K et al. Anion Transport Across Human Gallbladder Organoids and Monolayers. Front Physiol 13:882525 (2022). PubMed: 35685290
- Wang JL et al. Expression, Localization, and Effect of High Salt Intake on Electroneutral Na+/HCO3- Cotransporter NBCn2 in Rat Small Intestine: Implication in Intestinal NaCl Absorption. Front Physiol 10:1334 (2019). PubMed: 31736772
- Tingskov SJ et al. Tamoxifen Decreases Lithium-Induced Natriuresis in Rats With Nephrogenic Diabetes Insipidus. Front Physiol 9:903 (2018). PubMed: 30050465
- Wang YY et al. SLC9A3 Protein Is Critical for Acrosomal Formation in Postmeiotic Male Germ Cells. Int J Mol Sci 19:N/A (2017). PubMed: 29286340
- Wang YY et al. Loss of SLC9A3 decreases CFTR protein and causes obstructed azoospermia in mice. PLoS Genet 13:e1006715 (2017). IHC-P ; Mouse . PubMed: 28384194