Anti-SOD2/MnSOD抗体[mAbcam 74231] (ab74231)
Key features and details
- Mouse monoclonal [mAbcam 74231] to SOD2/MnSOD
- Suitable for: WB
- Knockout validated
- Reacts with: Rat, Human
- Isotype: IgG1
概述
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产品名称
Anti-SOD2/MnSOD抗体[mAbcam 74231]
参阅全部 SOD2/MnSOD 一抗 -
描述
小鼠单克隆抗体[mAbcam 74231] to SOD2/MnSOD -
宿主
Mouse -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt (Intra) -
种属反应性
与反应: Rat, Human
预测可用于: Mouse, Rabbit, Horse, Guinea pig, Cow, Pig, Chimpanzee, Zebrafish, Cynomolgus monkey, Macaque monkey, Orangutan -
免疫原
Synthetic peptide within Human SOD2/MnSOD aa 50-150 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: P04179 -
阳性对照
- WB: Recombinant human SOD2/MnSOD protein (ab93946), human liver; human brain; human heart; rat brain tissue lysates and Wild-type HEK-293T cell lysate.
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常规说明
This monoclonal antibody to SOD2 has been knockout validated in Western blot. The expected band for SOD2 was observed in wild type cells and the band was not seen in knockout cells.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
mAbcam 74231 -
骨髓瘤
Sp2/0-Ag14 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab74231于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 25 kDa).
We recommend using 3% milk as the blocking agent in Western Blot. |
说明 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 25 kDa). We recommend using 3% milk as the blocking agent in Western Blot. |
靶标
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功能
Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems. -
疾病相关
Genetic variation in SOD2 is associated with susceptibility to microvascular complications of diabetes type 6 (MVCD6) [MIM:612634]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. -
序列相似性
Belongs to the iron/manganese superoxide dismutase family. -
翻译后修饰
Nitrated under oxidative stress. Nitration coupled with oxidation inhibits the catalytic activity. -
细胞定位
Mitochondrion matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 6648 Human
- Entrez Gene: 20656 Mouse
- Entrez Gene: 24787 Rat
- Entrez Gene: 335799 Zebrafish
- Omim: 147460 Human
- SwissProt: P04179 Human
- SwissProt: P09671 Mouse
- SwissProt: P07895 Rat
see all -
别名
- Indophenoloxidase B antibody
- IPO B antibody
- IPOB antibody
see all
图片
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Lane 1: Wild-type HEK-293T cell lysate (40 ug)
Lane 2: Empty Lane
Lane 3: SOD2 knockout HEK-293T cell lysate (40 ug)Lanes 1 - 3: Merged signal (red and green). Green - ab74231 observed at 25 kDa. Orange - loading control, ab181602, observed at 37 kDa.
Gel type: MES
Blocking buffer: 3% Milkab74231 was shown to specifically react with SOD2. Wild-type and SOD2 knockout samples were subjected to SDS-PAGE. ab74231 and ab181602 GAPDH (loading control) were diluted 1 μg/mL and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.
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All lanes : Anti-SOD2/MnSOD antibody [mAbcam 74231] (ab74231) at 5 µg/ml
Lane 1 : Human liver tissue lysate - total protein (ab29889)
Lane 2 : Human heart tissue lysate - total protein (ab29431)
Lane 3 : Human brain tissue lysate - total protein (ab29466)
Lane 4 : Brain (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 25 kDa
Observed band size: 23 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutesThe Superoxide dismutase 2 protein has a predicted molecular weight of 25-kDa. However, the sequence contains a 24 residue transit peptide at the N-terminus and, when cleaved, the protein has an expected molecular weight of approximately 23-kDa.
We recommend using 3% milk as the blocking agent in Western Blot.
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Anti-SOD2/MnSOD antibody [mAbcam 74231] (ab74231) at 1 µg/ml + Recombinant Human SOD2/MnSOD protein (ab82656) at 0.01 µg
Secondary
Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 25 kDa
Exposure time: 12 minutes
数据表及文件
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Datasheet download
文献 (2)
ab74231 被引用在 2 文献中.
- Lin J et al. SIRT3 mitigates intervertebral disc degeneration by delaying oxidative stress-induced senescence of nucleus pulposus cells. J Cell Physiol 236:6441-6456 (2021). PubMed: 33565085
- Roos NJ et al. Lapatinib Activates the Kelch-Like ECH-Associated Protein 1-Nuclear Factor Erythroid 2-Related Factor 2 Pathway in HepG2 Cells. Front Pharmacol 11:944 (2020). PubMed: 32694997