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Full length protein corresponding to Rat SOD2/MnSOD.
Our Abpromise guarantee covers the use of ab13534 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|IHC-Fr||Use a concentration of 2 µg/ml.|
|ELISA||Use at an assay dependent concentration.|
|WB||1/2000. Detects a band of approximately 25 kDa (predicted molecular weight: 26.6 kDa).|
|IP||Use a concentration of 10 µg/ml.|
ab13534 staining SOD2 in Human colon and Horse Ileo tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/2000) for 18 hours.
This image is courtesy of an anonymous Abreview
ab13534 at 1/100 dilution staining Superoxide Dismutase 2 in mouse back skin tissue section by Immunohistochemistry (Bouin's fixative fixed paraffin-embedded tissue section). Antigen retrieval was done by microwave in citrate buffer. A Fluorophore-conjugated goat anti-rabbit secondary was used at 1/50 dilution.
Image courtesy of an anonymous Abreview.Primary antibody was incubated for 16 hours at 4°C, at a 1/2000 dilution.