, corresponding to C terminal amino acids 426-439 of Human SOCS7.
Principal Names – SOCS7; SOCS4; suppressor of cytokine signaling 7; SH2 domain containing SOCS box protein.
Official Gene Symbol - SOCS7.
GenBank Accession Number – NP_543143.
LocusLink ID - 122809 (human).
Gene Ontology terms - intracellular signaling cascade; regulation of cell growth.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, 5mg/ml Tris, pH 7.3
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 3 µg/ml.
Use a concentration of 3 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. May be a substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
Protein modification; protein ubiquitination.
Contains 1 SH2 domain.
Contains 1 SOCS box domain.
The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes.
Information by UniProt
Cytokine inducible SH2 protein 4 antibody
Western blot - SOCS4 antibody (ab3607)
ab3607 at a 2µg/ml concentration staining ~50 kDa SOCS 7 in NIH-3T3 cell lysate (RIPA buffer, 30µg total protein per lane) by Western blot. 1 hour incubation, ECL detection.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOCS4 antibody (ab3607)
ab3607 (3.8µg/ml) staining of paraffin embedded Human Pancreas shows cytoplasm staining on select beta cells in islet of Langerhans. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
has not yet been referenced specifically in any publications.
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