Anti-SOCS3抗体[5C2] (ab119806)

概述

  • 产品名称Anti-SOCS3抗体[5C2]
    参阅全部 SOCS3 一抗
  • 描述
    小鼠单克隆抗体[5C2] to SOCS3
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length protein of Human SOCS3 produced in HEK293T cells (NP_003946).

  • 阳性对照
    • HEK293T cells transfected with SOCS3, COS7 cells transiently transfected with SOCS3
  • 常规说明Dilute in PBS (pH7.3) before use.

性能

应用

Our Abpromise guarantee covers the use of ab119806 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000. Predicted molecular weight: 25 kDa.
ICC/IF 1/100.

靶标

  • 功能SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS3 is involved in negative regulation of cytokines that signal through the JAK/STAT pathway. Inhibits cytokine signal transduction by binding to tyrosine kinase receptors including gp130, LIF, erythropoietin, insulin, IL12, GCSF and leptin receptors. Binding to JAK2 inhibits its kinase activity. Suppresses fetal liver erythropoiesis. Regulates onset and maintenance of allergic responses mediated by T-helper type 2 cells. Regulates IL-6 signaling in vivo (By similarity). Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize IL6ST.
  • 组织特异性Widely expressed with high expression in heart, placenta, skeletal muscle, peripheral blood leukocytes, fetal and adult lung, and fetal liver and kidney. Lower levels in thymus.
  • 通路Protein modification; protein ubiquitination.
  • 疾病相关Note=There is some evidence that SOCS3 may be a susceptibility gene for atopic dermatitis linked to 17q25. SOCS3 messenger RNA is significantly more highly expressed in skin from patients with atopic dermatitis than in skin from healthy controls. Furthermore, a genetic association between atopic dermatitis and a haplotype in the SOCS3 gene has been found in two independent groups of patients.
  • 序列相似性Contains 1 SH2 domain.
    Contains 1 SOCS box domain.
  • 结构域The ESS and SH2 domains are required for JAK phosphotyrosine binding. Further interaction with the KIR domain is necessary for signal and kinase inhibition.
    The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes.
  • 翻译后修饰Phosphorylated on tyrosine residues after stimulation by the cytokines, IL-2, EPO or IGF1.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ATOD4 antibody
    • CIS 3 antibody
    • CIS-3 antibody
    • CIS3 antibody
    • Cish3 antibody
    • Cytokine induced SH2 protein 3 antibody
    • Cytokine-inducible SH2 protein 3 antibody
    • E2a Pbx1 target gene in fibroblasts 10 antibody
    • EF 10 antibody
    • MGC71791 antibody
    • SOCS 3 antibody
    • SOCS-3 antibody
    • Socs3 antibody
    • SOCS3_HUMAN antibody
    • SSI 3 antibody
    • SSI-3 antibody
    • SSI3 antibody
    • STAT induced STAT inhibitor 3 antibody
    • STAT-induced STAT inhibitor 3 antibody
    • Suppressor of cytokine signaling 3 antibody
    see all

Anti-SOCS3 antibody [5C2] 图像

  • All lanes : Anti-SOCS3 antibody [5C2] (ab119806) at 1/2000 dilution

    Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control
    Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY SOCS3

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 25 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC209305)
  • Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SOCS3 using ab119806 at a dilution of 1/100.

Anti-SOCS3 antibody [5C2] (ab119806)参考文献

ab119806 has not yet been referenced specifically in any publications.

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