Anti-SOCS2抗体(ab92847)

概述

  • 产品名称Anti-SOCS2抗体
    参阅全部 SOCS2 一抗
  • 描述
    兔多克隆抗体to SOCS2
  • 经测试应用适用于: WB, IP, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    A synthetic peptide corresponding to the C-terminus of SOCS2 protein from Human, Mouse and Rat origins.

  • 阳性对照
    • Mouse liver and muscle cell lysates. IHC-P: Human lung FFPE tissue sections.

性能

应用

Our Abpromise guarantee covers the use of ab92847 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
ELISA
IHC-P
  • 应用说明ELISA: Use at a concentration of 0.01 - 0.1 µg/ml.
    IHC-P: Use at a concentration of 0.5 - 2 µg/ml.
    IP: Use at a concentration of 2-5 µg/ml.
    WB: Use at a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 22 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS2 appears to be a negative regulator in the growth hormone/IGF1 signaling pathway. Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
    • 组织特异性High expression in heart, placenta, lung, kidney and prostate.
    • 通路Protein modification; protein ubiquitination.
    • 序列相似性Contains 1 SH2 domain.
      Contains 1 SOCS box domain.
    • 结构域The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CIS 2 antibody
      • CIS-2 antibody
      • CIS2 antibody
      • Cish 2 antibody
      • Cish2 antibody
      • Cytokine inducible SH2 protein 2 antibody
      • Cytokine-inducible SH2 protein 2 antibody
      • SOCS 2 antibody
      • SOCS-2 antibody
      • Socs2 antibody
      • SOCS2_HUMAN antibody
      • SSI 2 antibody
      • SSI-2 antibody
      • SSI2 antibody
      • STAT induced STAT inhibitor 2 antibody
      • STAT-induced STAT inhibitor 2 antibody
      • STATI 2 antibody
      • STATI2 antibody
      • Suppressor of cytokine signaling 2 antibody
      see all

    Anti-SOCS2 antibody 图像

    • IHC image of SOCS2 staining in human lung formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab92847, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • All lanes : Anti-SOCS2 antibody (ab92847) at 1/500 dilution

      Lane 1 : Mouse liver cell Lysate
      Lane 2 : Mouse muscle cell Lysate


      Predicted band size : 22 kDa
      Observed band size : 22 kDa

    Anti-SOCS2 antibody (ab92847)参考文献

    ab92847 has not yet been referenced specifically in any publications.

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (tumor colon cells)
    Loading amount 25 µg
    Specification tumor colon cells
    Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

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    Verified customer

    提交于 Sep 12 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"