概述

  • 产品名称Anti-SOCS2抗体
    参阅全部 SOCS2 一抗
  • 描述
    兔多克隆抗体to SOCS2
  • 经测试应用适用于: IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to the C terminal region of human SOCS2.

  • 阳性对照
    • Human pancreas IF/ICC: HepG2 cell line.

性能

应用

Our Abpromise guarantee covers the use of ab74533 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P
ICC/IF
  • 应用说明IHC-P: 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS2 appears to be a negative regulator in the growth hormone/IGF1 signaling pathway. Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
    • 组织特异性High expression in heart, placenta, lung, kidney and prostate.
    • 通路Protein modification; protein ubiquitination.
    • 序列相似性Contains 1 SH2 domain.
      Contains 1 SOCS box domain.
    • 结构域The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CIS 2 antibody
      • CIS-2 antibody
      • CIS2 antibody
      • Cish 2 antibody
      • Cish2 antibody
      • Cytokine inducible SH2 protein 2 antibody
      • Cytokine-inducible SH2 protein 2 antibody
      • SOCS 2 antibody
      • SOCS-2 antibody
      • Socs2 antibody
      • SOCS2_HUMAN antibody
      • SSI 2 antibody
      • SSI-2 antibody
      • SSI2 antibody
      • STAT induced STAT inhibitor 2 antibody
      • STAT-induced STAT inhibitor 2 antibody
      • STATI 2 antibody
      • STATI2 antibody
      • Suppressor of cytokine signaling 2 antibody
      see all

    Anti-SOCS2 antibody 图像

    • ICC/IF image of ab74533 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab74533, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Human pancreas stained with ab74533 at 1/100 dilution.

    Anti-SOCS2 antibody (ab74533)参考文献

    This product has been referenced in:
    • Iglesias-Gato D  et al. SOCS2 mediates the cross talk between androgen and growth hormone signaling in prostate cancer. Carcinogenesis N/A:N/A (2013). Read more (PubMed: 24031028) »
    • de Andrés MC  et al. Suppressors of cytokine signalling (SOCS) are reduced in osteoarthritis. Biochem Biophys Res Commun : (2011). IHC-P ; Human . Read more (PubMed: 21352802) »

    See all 2 Publications for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Dog Tissue sections (liver)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: tris-EDTA, pH9.0
    Permeabilization No
    Specification liver
    Blocking step Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative Formaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 Mar 03 2016

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Dog Tissue sections (liver)
    Permeabilization No
    Specification liver
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative Acetone
    Username

    Abcam user community

    Verified customer

    提交于 Feb 19 2016

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (nerve)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH6.0
    Permeabilization No
    Specification nerve
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative Formaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 Sep 22 2015

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10 mM citrate buffer, pH6.0
    Sample Rat Tissue sections (sciatic nerve)
    Specification sciatic nerve
    Permeabilization No
    Fixative Formaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 Aug 28 2014

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