概述

  • 产品名称Anti-SOCS2抗体
    参阅全部 SOCS2 一抗
  • 描述
    兔多克隆抗体to SOCS2
  • 特异性By Western blot, this antibody detects a band of approximately 23 kDa in lysate of GH treated HEK 293 cells transfected with GHR, which corresponds to the predicted molecular weight of SOCS 2.
  • 经测试应用适用于: WB, IP, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (

    PTRLKDYLEEYKFQV

    ) corresponding to amino acids 184-198 of human, rat and mouse SOCS 2.

  • 阳性对照
    • lysate of GH treated HEK 293 cells transfected with GHR, E17 mouse cortical neuron lysate (see Scott et al)
  • 常规说明


    Accumulating evidence demonstrates that cytokine receptor signaling is negatively regulated by a family of Src homology 2 domain-containing adaptor molecules Termed SOCS (Suppressor of Cytokine Signaling). To date, there are eight members of SOCS family have been recognized, they are SOCS 1, 2, 3, 4, 5, 6, 7 and CIS. Structurally the SOCS proteins are composed of an N-terminal region of variable length and amino acid composition, a central SH2 domain, and a previously unrecognized C-terminal motif that we have called the SOCS box. The SOCS proteins appear to form part of a classical negative feed back loop that regulates cytokine signal transduction via a STAT induced transcriptional mechanism. Transcription of each of the SOCS genes occurs rapidly in vitro and in vivo in response to cytokines, and once produced, the various members of the SOCS family appear to inhibit signaling in different ways. SOCS 2, not like SOCS 1 and SOCS 3, regulates postnatal growth, likely through its ability to influence the GH / IGF 1axis, although they might have overlapping actions.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液PBS with 0.02% sodium azide
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • Primary antibody说明Accumulating evidence demonstrates that cytokine receptor signaling is negatively regulated by a family of Src homology 2 domain-containing adaptor molecules Termed SOCS (Suppressor of Cytokine Signaling). To date, there are eight members of SOCS family have been recognized, they are SOCS 1, 2, 3, 4, 5, 6, 7 and CIS. Structurally the SOCS proteins are composed of an N-terminal region of variable length and amino acid composition, a central SH2 domain, and a previously unrecognized C-terminal motif that we have called the SOCS box. The SOCS proteins appear to form part of a classical negative feed back loop that regulates cytokine signal transduction via a STAT induced transcriptional mechanism. Transcription of each of the SOCS genes occurs rapidly in vitro and in vivo in response to cytokines, and once produced, the various members of the SOCS family appear to inhibit signaling in different ways. SOCS 2, not like SOCS 1 and SOCS 3, regulates postnatal growth, likely through its ability to influence the GH / IGF 1axis, although they might have overlapping actions.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab3692 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
IP Use 3-5µg for 107 cells.
ELISA Use a concentration of 0.1 - 1 µg/ml.

靶标

  • 功能SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS2 appears to be a negative regulator in the growth hormone/IGF1 signaling pathway. Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
  • 组织特异性High expression in heart, placenta, lung, kidney and prostate.
  • 通路Protein modification; protein ubiquitination.
  • 序列相似性Contains 1 SH2 domain.
    Contains 1 SOCS box domain.
  • 结构域The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CIS 2 antibody
    • CIS-2 antibody
    • CIS2 antibody
    • Cish 2 antibody
    • Cish2 antibody
    • Cytokine inducible SH2 protein 2 antibody
    • Cytokine-inducible SH2 protein 2 antibody
    • SOCS 2 antibody
    • SOCS-2 antibody
    • Socs2 antibody
    • SOCS2_HUMAN antibody
    • SSI 2 antibody
    • SSI-2 antibody
    • SSI2 antibody
    • STAT induced STAT inhibitor 2 antibody
    • STAT-induced STAT inhibitor 2 antibody
    • STATI 2 antibody
    • STATI2 antibody
    • Suppressor of cytokine signaling 2 antibody
    see all

Anti-SOCS2 antibody 图像



  • Predicted band size : 23 kDa

    Image from Gilli F et al., PLoS One. 2010 Jan 29;5(1):e8962. Fig 4.; doi:10.1371/journal.pone.0008962; PLoS ONE 5(1): e8962.

Anti-SOCS2 antibody (ab3692)参考文献

This product has been referenced in:
  • Gurda GT  et al. Profiling CCK-mediated pancreatic growth: the dynamic genetic program and the role of STATs as potential regulators. Physiol Genomics 44:14-24 (2012). WB ; Mouse . Read more (PubMed: 22010007) »
  • Gilli F  et al. Learning from nature: pregnancy changes the expression of inflammation-related genes in patients with multiple sclerosis. PLoS One 5:e8962 (2010). WB ; Human . Read more (PubMed: 20126412) »

See all 9 Publications for this product

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (macrophage)
Specification macrophage
Treatment 0.1 ug/ml LPS 24 h
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Dec 22 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 50 cells
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (primary human macrophages)
Specification primary human macrophages
Treatment MDP 100ug/ml
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Dr. sha zheng

Verified customer

提交于 Jun 18 2013

Thank you for passing on this enquiry from the customer. I hope you are well. All our antibodies that have been tested western blotting will detect reduced proteins, unless otherwise stated the datasheet. The customer will therefore need to reduce ...

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Thank you for getting back to me with your customer's further information. Please can you pass on the following comments to your customer: I am surprised that you have been unable to detect consistent hypothalamus staining using this antibody. It i...

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Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. Quality is very important to Abcam. We take all of our complaints very seriously and will not hesitate to remove an antibody should we have co...

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Thank you for your enquiry. Unfortunately we do no have a recommended positive control for SOCS1 antibody (ab3691). However, the customer that left a favourable Abreview used a human cell lysate - whole cell (human malignant melanoma cells). Furthe...

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I have received feedback from the source of ab3692 stating that unfortunately they do not know if the cells were transfected with the receptor or not. We do not have the stimulation paradigm information unfortunately, I'm very sorry. From your literatu...

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