Anti-SOCS1抗体- Carboxyterminal end (ab65989)


  • 产品名称Anti-SOCS1抗体- Carboxyterminal end
    参阅全部 SOCS1 一抗
  • 描述
    兔多克隆抗体to SOCS1 - Carboxyterminal end
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to a sequence at the C-terminal of human SOCS1, identical to the related mouse and rat sequence.

  • 阳性对照
    • Rat lung tissue.




Our Abpromise guarantee covers the use of ab65989 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 24 kDa.
IHC-P Use a concentration of 0.5 - 1 µg/ml.


  • 功能SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS1 is involved in negative regulation of cytokines that signal through the JAK/STAT3 pathway. Through binding to JAKs, inhibits their kinase activity. In vitro, also suppresses Tec protein-tyrosine activity. Appears to be a major regulator of signaling by interleukin 6 (IL6) and leukemia inhibitory factor (LIF). Regulates interferon-gamma mediated sensory neuron survival (By similarity). Probable substrate recognition component of an ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize JAK2.
  • 组织特异性Expressed in all tissues with high expression in spleen, small intestine and peripheral blood leukocytes.
  • 通路Protein modification; protein ubiquitination.
  • 序列相似性Contains 1 SH2 domain.
    Contains 1 SOCS box domain.
  • 结构域The ESS and SH2 domains are required for JAK phosphotyrosine binding. Further interaction with the KIR domain is necessary for signal and kinase inhibition.
    The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes. The Elongin BC complex binding domain is also known as BC-box with the consensus [APST]-L-x(3)-C-x(3)-[AILV] and is part of the SOCS box.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CISH 1 antibody
    • CISH1 antibody
    • Cytokine inducible SH2 protein 1 antibody
    • JAB antibody
    • JAK binding protein antibody
    • JAK-binding protein antibody
    • Janus kinase binding protein antibody
    • SOCS 1 antibody
    • SOCS-1 antibody
    • Socs1 antibody
    • SOCS1_HUMAN antibody
    • SSI 1 antibody
    • SSI-1 antibody
    • SSI1 antibody
    • STAT induced STAT inhibitor 1 antibody
    • STAT-induced STAT inhibitor 1 antibody
    • Suppressor of cytokine signaling 1 antibody
    • Supressor of cytokine signalling 1 antibody
    • TEC interacting protein 3 antibody
    • Tec-interacting protein 3 antibody
    • TIP 3 antibody
    • TIP-3 antibody
    • TIP3 antibody
    see all

Anti-SOCS1 antibody - Carboxyterminal end 图像

  • ab65989, at 0.5µg/ml, staining SOCS1 in paraffin-embedded rat lung tissue by immunohistochemistry. Staining of SOCS1 in the nucleus is observed. DAB chromogenic reaction.
  • All lanes : Anti-SOCS1 antibody - Carboxyterminal end (ab65989) at 1 µg/ml

    Lane 1 : Human spleen tissue lysate - total protein (ab29699)
    Lane 2 : Human small intestine tissue lysate - total protein (ab29276)

    Lysates/proteins at 10 µg per lane.

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 24 kDa
    Observed band size : 32 kDa (why is the actual band size different from the predicted?)

    Exposure time : 30 seconds

Anti-SOCS1 antibody - Carboxyterminal end (ab65989)参考文献

This product has been referenced in:
  • Muratsu D  et al. Zoledronic acid enhances lipopolysaccharide-stimulated proinflammatory reactions through controlled expression of SOCS1 in macrophages. PLoS One 8:e67906 (2013). WB . Read more (PubMed: 23874464) »

See 1 Publication for this product

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To our knowledge, customised protocols are not required for this product, any general Flow Cytometry protocol could be used with this antibody.

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