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A synthetic peptide corresponding to C terminal of SOCS1 protein from Human and Mouse origins.
Our Abpromise guarantee covers the use of ab83493 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).|
|IP||Use a concentration of 2 - 5 µg/ml.|
|ELISA||Use a concentration of 0.01 - 0.1 µg/ml.|
|IHC-P||Use a concentration of 0.5 - 2 µg/ml.|
|ICC/IF||Use a concentration of 5 µg/ml.|
IHC image of SOCS1 staining in Human normal spleen formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab83493, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times
ab83493 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"