概述

  • 产品名称
  • 描述
    兔多克隆抗体to SOAT 1
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Cow, Cynomolgus monkey
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 500 to the C-terminus of Human SOAT 1.

  • 阳性对照
    • This antibody gave a positive signal in the following lysates: Mouse Spleen Tissue; Ramos Whole Cell; RAW264.7 Whole Cell (data not shown).

性能

应用

Our Abpromise guarantee covers the use of ab72229 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 65 kDa).

靶标

  • 功能
    Catalyzes the formation of fatty acid-cholesterol esters. Plays a role in lipoprotein assembly and dietary cholesterol absorption. In addition to its acyltransferase activity, it may act as a ligase.
  • 序列相似性
    Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily.
  • 细胞定位
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ACACT antibody
    • ACAT antibody
    • ACAT-1 antibody
    • ACAT1 antibody
    • Acyl coenzyme A cholesterol acyltransferase 1 antibody
    • Acyl-coenzyme A:cholesterol acyltransferase 1 antibody
    • Cholesterol acyltransferase 1 antibody
    • SOAT antibody
    • SOAT1 antibody
    • SOAT1_HUMAN antibody
    • STAT antibody
    • Sterol O acyltransferase 1 antibody
    • Sterol O-acyltransferase 1 antibody
    see all

图片

  • All lanes : Anti-SOAT 1 antibody (ab72229) at 1 µg/ml

    Lane 1 : Spleen (Mouse) Tissue Lysate
    Lane 2 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under non-reducing conditions.

    Predicted band size : 65 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 28 kDa,37 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes

实验方案

文献

ab72229 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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