兔多克隆抗体to SOAT 1
Rat, Cow, Cynomolgus monkey
Synthetic peptide conjugated to KLH derived from within residues 500 to the C-terminus of Human SOAT 1.
- This antibody gave a positive signal in the following lysates: Mouse Spleen Tissue; Ramos Whole Cell; RAW264.7 Whole Cell (data not shown).
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 65 kDa).
Catalyzes the formation of fatty acid-cholesterol esters. Plays a role in lipoprotein assembly and dietary cholesterol absorption. In addition to its acyltransferase activity, it may act as a ligase.
Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily.
Endoplasmic reticulum membrane.
Information by UniProt
- ACACT antibody
- ACAT antibody
- ACAT-1 antibody
Western blot - SOAT 1 antibody (ab72229)
All lanes :
Anti-SOAT 1 antibody (ab72229) at 1 µg/mlLane 1 :
Spleen (Mouse) Tissue Lysate Lane 2 :
Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.SecondaryAll lanes :
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under non-reducing conditions.Predicted band size:
65 kDaObserved band size:
55 kDa (why is the actual band size different from the predicted?
)Additional bands at:
28 kDa, 37 kDa. We are unsure as to the identity of these extra bands.Exposure time:
The 55 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to SOAT1.
has not yet been referenced specifically in any publications.
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