概述

  • 产品名称
  • 描述
    山羊多克隆抗体to SNX5
  • 经测试应用
    适用于: IHC-P, ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Cow, Dog, Pig
  • 免疫原

    Synthetic peptide:

    SLLQSCIDLFKNN

    , corresponding to C terminal amino acids 392-404 of Human SNX5.

  • 阳性对照
    • Jurkat Lysate.
  • 常规说明
    GenBank Accession Number – NP_055241; NP_689413

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris-saline. pH 7.3
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab5983 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 3 - 6 µg/ml.
ICC/IF Use at an assay dependent dilution. PubMed: 20065094
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).

靶标

  • 功能
    May be involved in several stages of intracellular trafficking. Plays a role in macropinocytosis. Plays a role in the internalization of EGFR after EGF stimulation.
  • 序列相似性
    Belongs to the sorting nexin family.
    Contains 1 BAR domain.
    Contains 1 PX (phox homology) domain.
  • 结构域
    The PX domain mediates interaction with membranes enriched in phosphatidylinositol 3,4-bisphosphate and/or phosphatidylinositol 4,5-bisphosphate.
  • 细胞定位
    Early endosome membrane. Cell membrane. Cytoplasmic vesicle membrane. Cytoplasm. Cell projection > phagocytic cup. Cell projection > ruffle. Recruited to the plasma membrane after EGF stimulation, which leads to increased levels of phosphatidylinositol 3,4-bisphosphate (PdtIns(3,4)P2). Detected on macropinosomes. Targeted to membrane ruffles in response to EGFR stimulation.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 0910001N05Rik antibody
    • 1810032P22Rik antibody
    • AU019504 antibody
    • D2Ertd52e antibody
    • FLJ10931 antibody
    • OTTHUMP00000030340 antibody
    • OTTHUMP00000062927 antibody
    • OTTMUSP00000003367 antibody
    • OTTMUSP00000003368 antibody
    • RP11-504H3.2 antibody
    • RP23-35E16.2 antibody
    • snoRNA MBI-43 antibody
    • SNX 5 antibody
    • SNX5 antibody
    • SNX5_HUMAN antibody
    • Sorting nexin 5 antibody
    • Sorting nexin-5 antibody
    see all

图片

  • Anti-SNX5 antibody (ab5983) at 1 µg/ml + Jurkat cell lysate (in RIPA buffer) at 35 µg

    Predicted band size : 47 kDa
    Observed band size : 47 kDa
  • ab5983 at 4µg/ml, staining SNX5 in Human breast tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with pH 6 citrate buffer was performed, and the sample was stained with HRP.
  • HeLa cells were grown on coverslips, washed in warm PBS, fixed in warm PFA (3.7% in PBS, 10 minutes), then washed (2x, 5 minutes, PBS), permeabilized (4 minutes, 3% BSA, and 0.5% Triton X-100 in PBS), and blocked in blocking solution (3% BSA in PBS, 30 minutes). Antibody labeling was performed by inversion of coverslips on 20 µl blocking solution with primary or secondary antibodies (1–5 µg/ml).
  • Anti-SNX5 antibody (ab5983) at 1/500 dilution + whole cell lysate prepared from murine macrophages treated with INF gamma at 20 µg

    Secondary
    HRP conjugated rabbit anti-goat polyclonal at 1/5000 dilution
    Developed using the ECL technique

    Predicted band size : 47 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    Image courtesy of an anonymous Abreview.

    See Abreview

文献

This product has been referenced in:
  • Halamoda-Kenzaoui B  et al. The agglomeration state of nanoparticles can influence the mechanism of their cellular internalisation. J Nanobiotechnology 15:48 (2017). WB ; Human . Read more (PubMed: 28651541) »
  • Steinberg F  et al. A global analysis of SNX27-retromer assembly and cargo specificity reveals a function in glucose and metal ion transport. Nat Cell Biol 15:461-71 (2013). Human . Read more (PubMed: 23563491) »

See all 3 Publications for this product

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (macrophage)
Loading amount
20 µg
Specification
macrophage
Treatment
IFNgamma
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Mar 01 2012

Thank you for your enquiry. I'm afraid we don't sell the immunogen/peptide for this antibody. Please contact us again if you have further questions. Best of luck with your research.

Thank you for your enquiry and I'm sorry to hear that you are experiencing difficulty with ab5983. At this point I would like to make the following suggestions in order to help improve your results. Decrease the amount of secondary antibody that you ar...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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