antigen sequence: VGGDSTPSPD LLMARSFGDK DLILPNGGTP AGTSSPASSS SLLNRLQLDD DIDGETRDLF VIVDDPKKHV CTMETY, corresponding to amino acids 33-108 of Human SNX30.
- Human duodenum tissue and RT4, U 251 MG, Human Liver and Tonsil lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||1/250 - 1/500.
May be involved in several stages of intracellular trafficking.
Belongs to the sorting nexin family.
Contains 1 PX (phox homology) domain.
Information by UniProt
- snx30 antibody
- SNX30_HUMAN antibody
- Sorting nexin-30 antibody
Immunocytochemistry/ Immunofluorescence - Anti-SNX30 antibody (ab121600)
Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus, nucleoli and cytoplasm. Recommended concentration of ab121600 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX30 antibody (ab121600)
ab121600 at 1/125 dilution staining SNX30 in Paraffin-embedded Human Duodenum tissue by Immunohistochemistry.
Note: strong cytoplasmic positivity in glandular cells.
Western blot - Anti-SNX30 antibody (ab121600)
All lanes : Anti-SNX30 antibody (ab121600) at 1/250 dilution
Lane 1 : RT4 cell lysate
Lane 2 : U 251 MG cell lysate
Lane 3 : Human Plasma
Lane 4 : Human Liver lysate
Lane 5 : Human Tonsil lysate
Developed using the ECL technique
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"