Anti-SNX30抗体(ab121600)
Key features and details
- Rabbit polyclonal to SNX30
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-SNX30抗体 -
描述
兔多克隆抗体to SNX30 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, IHC-P, WBmore details -
种属反应性
与反应: Human -
免疫原
antigen sequence: VGGDSTPSPD LLMARSFGDK DLILPNGGTP AGTSSPASSS SLLNRLQLDD DIDGETRDLF VIVDDPKKHV CTMETY, corresponding to amino acids 33-108 of Human SNX30.
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阳性对照
- Human duodenum tissue and RT4, U 251 MG, Human Liver and Tonsil lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab121600于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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说明 |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
靶标
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功能
May be involved in several stages of intracellular trafficking. -
序列相似性
Belongs to the sorting nexin family.
Contains 1 PX (phox homology) domain. - Information by UniProt
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数据库链接
- Entrez Gene: 401548 Human
- SwissProt: Q5VWJ9 Human
- Unigene: 522350 Human
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别名
- snx30 antibody
- SNX30_HUMAN antibody
- Sorting nexin-30 antibody
图片
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Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus, nucleoli and cytoplasm. Recommended concentration of ab121600 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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ab121600 at 1/125 dilution staining SNX30 in Paraffin-embedded Human Duodenum tissue by Immunohistochemistry.
Note: strong cytoplasmic positivity in glandular cells. -
All lanes : Anti-SNX30 antibody (ab121600) at 1/250 dilution
Lane 1 : RT4 cell lysate
Lane 2 : U 251 MG cell lysate
Lane 3 : Human Plasma
Lane 4 : Human Liver lysate
Lane 5 : Human Tonsil lysate
Developed using the ECL technique.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab121600 被引用在 2 文献中.
- Antón Z et al. A heterodimeric SNX4--SNX7 SNX-BAR autophagy complex coordinates ATG9A trafficking for efficient autophagosome assembly. J Cell Sci 133:N/A (2020). PubMed: 32513819
- Simonetti B et al. Sequence-dependent cargo recognition by SNX-BARs mediates retromer-independent transport of CI-MPR. J Cell Biol 216:3695-3712 (2017). PubMed: 28935633