概述

  • 产品名称Anti-SNX3抗体
    参阅全部 SNX3 一抗
  • 描述
    兔多克隆抗体to SNX3
  • 经测试应用适用于: WB, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Human, Recombinant Fragment
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide (Human) sequence C-terminal end.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.01% Sodium Azide
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab56078 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 19 kDa. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
ELISA Use at an assay dependent dilution.
ICC/IF Use a concentration of 1 - 5 µg/ml.

靶标

  • 功能Phosphoinositide-binding protein required for multivesicular body formation. Specifically binds phosphatidylinositol-3-phosphate (PtdIns(P3)). Plays a role in protein transport between cellular compartments. Promotes stability and cell surface expression of epithelial sodium channel (ENAC) subunits SCNN1A and SCNN1G (By similarity). Not involved in EGFR degradation.
  • 疾病相关A chromosomal aberration involving SNX3 may be a cause of microphthalmia syndromic type 8 (MCOPS8) [MIM:601349]. Translocation t(6;13)(q21;q12). Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS8 is a very rare congenital syndrome characterized by microcephaly, microphthalmia, ectrodactyly of the lower limbs and prognathism. Intellectual deficit has been reported.
  • 序列相似性Belongs to the sorting nexin family.
    Contains 1 PX (phox homology) domain.
  • 结构域The PX domain mediates specific binding to phosphatidylinositol-3-phosphate (PtdIns(P3)).
  • 翻译后修饰Ubiquitinated, leading to its proteasomal degradation. Deubiquitinated by USP10.
  • 细胞定位Early endosome.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Grd19 antibody
    • MCOPS8 antibody
    • MGC151262 antibody
    • MGC151266 antibody
    • MGC17570 antibody
    • OTTHUMP00000016941 antibody
    • OTTHUMP00000046475 antibody
    • OTTHUMP00000046476 antibody
    • Protein SDP3 antibody
    • SDP3 antibody
    • SNX3 antibody
    • SNX3_HUMAN antibody
    • SNX3A antibody
    • Sorting nexin 3 antibody
    • Sorting nexin 3A antibody
    • Sorting nexin-3 antibody
    see all

Anti-SNX3 antibody 图像

  • ICC/IF image of ab56078 stained Hek293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab56078, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-SNX3 antibody (ab56078)参考文献

This product has been referenced in:
  • Morel E  et al. Phosphatidylinositol-3-phosphate regulates sorting and processing of amyloid precursor protein through the endosomal system. Nat Commun 4:2250 (2013). Read more (PubMed: 23907271) »

See 1 Publication for this product

Product Wall

Application Western blot
Loading amount 40 µg
Gel Running Conditions Reduced Denaturing (4-20% gradient gel)
Sample Mouse Cell lysate - whole cell (MEF, mouse Raw264 cells)
Specification MEF, mouse Raw264 cells
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
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提交于 Sep 15 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"