The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at a concentration of 10 µg/ml. Note: the antibody requires purification with protein A chromatography prior to use for ICC/IF.
WB: 1/500 - 1/1000. Detects a band of approximately 145 kDa (predicted molecular weight: 112 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
SNX13 is a negative regulator of signal transduction with a phosphoinositide binding domain, and may be involved in several stages of intracellular trafficking.