SNX1 overexpression 293T裂解液(全细胞) (ab94160)

概述

  • 产品名称SNX1 overexpression 293T裂解液(全细胞)
    参阅全部 SNX1 细胞裂解液
  • 描述
    SNX1 overexpression裂解液
  • 常规说明ab94160 is a 293T cell transfected lysate in which Human SNX1 has been transiently over-expressed using a pCMV-SNX1 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view the preparation notes.
  • 经测试应用适用于: WBmore details

性能

  • 背景Function: May be involved in several stages of intracellular trafficking. Plays a role in targeting ligand-activated EGFR to the lysosomes for degradation after endocytosis from the cell surface and release from the Golgi. Component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Interacts with membranes containing phosphatidylinositol 3-phosphate (PtdIns(3P)) or phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Similarity: Belongs to the sorting nexin family. Contains 1 BAR domain. Contains 1 PX (phox homology) domain.

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应用

Our Abpromise guarantee covers the use of ab94160 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution.

SNX1 overexpression 293T lysate (whole cell) 图像

  • ab94160 at 15 µg/lane on an SDS-PAGE gel
  • All lanes : Anti-SNX1 antibody (ab55776) at 1/500 dilution

    Lane 1 : SNX1 overexpression 293T lysate (whole cell) (ab94160)
    Lane 2 : 293T non-transfected Lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

SNX1 overexpression 293T lysate (whole cell) (ab94160)参考文献

ab94160 has not yet been referenced specifically in any publications.

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