SNX1 overexpression 293T裂解液(全细胞) (ab94159)

Submit a Question
Customer reviews and FAQs

概述

  • 产品名称
    SNX1 overexpression 293T裂解液(全细胞)
    参阅全部 SNX1 细胞裂解液
  • 描述
    SNX1 overexpression裂解液
  • 常规说明
    ab94159 is a 293T cell transfected lysate in which Human SNX1 has been transiently over-expressed using a pCMV-SNX1 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view the preparation notes.
  • 经测试应用
    适用于: WBmore details

性能

  • 背景
    Function: May be involved in several stages of intracellular trafficking. Plays a role in targeting ligand-activated EGFR to the lysosomes for degradation after endocytosis from the cell surface and release from the Golgi. Component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Interacts with membranes containing phosphatidylinositol 3-phosphate (PtdIns(3P)) or phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Similarity: Belongs to the sorting nexin family. Contains 1 BAR domain. Contains 1 PX (phox homology) domain.

应用

Our Abpromise guarantee covers the use of ab94159 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution.

SNX1 overexpression 293T lysate (whole cell) 图像

  • SDS-PAGE - SNX1 293T Transfected Lysate - (positive control) (ab94159)
    SDS-PAGE - SNX1 293T Transfected Lysate - (positive control) (ab94159)
    ab94159 at 15 µg/lane on an SDS-PAGE gel
  • Western blot - SNX1 293T Transfected Lysate - (positive control) (ab94159)
    Western blot - SNX1 293T Transfected Lysate - (positive control) (ab94159)
    All lanes : Anti-SNX1 antibody (ab55776) at 1/500 dilution

    Lane 1 : SNX1 overexpression 293T lysate (whole cell) (ab94159)
    Lane 2 : 293T non-transfected Lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

SNX1 overexpression 293T lysate (whole cell) (ab94159)参考文献

ab94159 has not yet been referenced specifically in any publications.

发表研究结果有使用 ab94159？请让我们知道，以便我们可以引用本数据表中的参考文章。

Product Wall

提交 ab94159 的问题

There are currently no Questions for ab94159.
Please use the link above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册