概述
-
产品名称SNX1 overexpression 293T裂解物(whole cell)
参阅全部 SNX1 细胞裂解液 -
常规说明
ab94159 is a 293T cell transfected lysate in which Human SNX1 has been transiently over-expressed using a pCMV-SNX1 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view the preparation notes.
-
经测试应用适用于: WBmore details
性能
-
形式Liquid
-
存放说明Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
-
存储溶液pH: 6.80
Constituent: 100% 1x Sample Buffer -
Concentration information loading... -
纯度Whole Cell Lysate
-
研究领域
-
背景Function: May be involved in several stages of intracellular trafficking. Plays a role in targeting ligand-activated EGFR to the lysosomes for degradation after endocytosis from the cell surface and release from the Golgi. Component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Interacts with membranes containing phosphatidylinositol 3-phosphate (PtdIns(3P)) or phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Similarity: Belongs to the sorting nexin family. Contains 1 BAR domain. Contains 1 PX (phox homology) domain.
应用
Our Abpromise guarantee covers the use of ab94159 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use at an assay dependent dilution. |
图片
-
SDS-PAGE - SNX1 293T Transfected Lysate - (positive control) (ab94159)ab94159 at 15 µg/lane on an SDS-PAGE gel -
Western blot - SNX1 293T Transfected Lysate - (positive control) (ab94159)All lanes : Anti-SNX1 antibody (ab55776) at 1/500 dilution
Lane 1 :SNX1 overexpression 293T lysate (whole cell) (ab94159)
Lane 2 : 293T non-transfected Lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
文献
ab94159 has not yet been referenced specifically in any publications.
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"