概述
- 产品名称SNX1 overexpression 293T裂解液(全细胞)
参阅全部 SNX1 细胞裂解液 - 描述SNX1 overexpression裂解液
- 常规说明ab94159 is a 293T cell transfected lysate in which Human SNX1 has been transiently over-expressed using a pCMV-SNX1 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view the preparation notes.
- 经测试应用适用于: WBmore details
性能
- 形式Liquid
- 存放说明Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
- 存储溶液pH: 6.80
Constituent: 100% 1x Sample Buffer -
Concentration information loading... - 纯度Whole Cell Lysate
- 研究领域
- 背景Function: May be involved in several stages of intracellular trafficking. Plays a role in targeting ligand-activated EGFR to the lysosomes for degradation after endocytosis from the cell surface and release from the Golgi. Component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Interacts with membranes containing phosphatidylinositol 3-phosphate (PtdIns(3P)) or phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Similarity: Belongs to the sorting nexin family. Contains 1 BAR domain. Contains 1 PX (phox homology) domain.
应用
Our Abpromise guarantee covers the use of ab94159 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use at an assay dependent dilution. |
SNX1 overexpression 293T lysate (whole cell) 图像
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SDS-PAGE - SNX1 293T Transfected Lysate - (positive control) (ab94159)ab94159 at 15 µg/lane on an SDS-PAGE gel -
Western blot - SNX1 293T Transfected Lysate - (positive control) (ab94159)All lanes : Anti-SNX1 antibody (ab55776) at 1/500 dilution
Lane 1 :SNX1 overexpression 293T lysate (whole cell) (ab94159)
Lane 2 : 293T non-transfected Lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
SNX1 overexpression 293T lysate (whole cell) (ab94159)参考文献
ab94159 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"