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Our Abpromise guarantee covers the use of ab99286 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 59 kDa). An approximately 75 kDa band is observed in Human lysates of the cell line HeLa. The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources.|
|ICC/IF||Use a concentration of 3 - 5 µg/ml.|
ab99286 staining SNX1 in SH5Y5Y cells by Immunocytochemistry/ Immunofluorescence. ab99286 diluted to 5 μg/ml showing vesicular staining.
99286 (3.8µg/ml) staining of paraffin embedded Human Uterus tissue following Steamed antigen retrieval with citrate buffer pH 6 and AP-staining shows vesicular staining in the cytoplasm of endometrial epithelium.
ab99286 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"