Mouse, Rat, Horse, Chicken, Cow, Pig, Chimpanzee, Zebrafish, Macaque monkey, Orangutan
Synthetic peptide corresponding to Human SNRPG aa 1 to the C-terminus conjugated to Keyhole Limpet Haemocyanin (KLH).
Database link: P62308
This antibody gave a positive signal in HEK293 whole cell lysate.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide Constituent: PBS Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
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Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 8 kDa (predicted molecular weight: 8 kDa).
Appears to function in the U7 snRNP complex that is involved in histone 3'-end processing. Associated with snRNP U1, U2, U4/U6 and U5.
Belongs to the snRNP Sm proteins family.
Information by UniProt
Sm protein G antibody
Western blot - Anti-SNRPG antibody (ab111194)
Anti-SNRPG antibody (ab111194) at 1 µg/ml (Milk blocking 3%) + HEK293 (Human) Whole Cell Lysate (
) at 25 µg
Goat Anti-Rabbit IgG H&L (HRP) (
) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size:
Observed band size:
Additional bands at:
36 kDa (possible non-specific binding), 80 kDa (possible non-specific binding)
This blot was produced using a 10% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab111194 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
has not yet been referenced specifically in any publications.
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