Anti-SNRPD3抗体(ab111094)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to SNRPD3
  • 经测试应用
    适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Cow, Dog, Pig, Chimpanzee, Zebrafish, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to Human SNRPD3 aa 1-100 conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: P62318

  • 阳性对照
    • This antibody gave a positive signal in Jurkat whole cell lysate and HeLa Nuclear lysate.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab111094 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 14 kDa).
ICC/IF 1/500.

靶标

图片

  • ab111094 staining HeLa cells by ICC/IF. The cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton X100 in PBS. The cells were then stained with ab111094 at 1/500 in PBS for 1h at 22°C. An Alexa Fluro 488 goat anti-rabbit polyclonal antibody at 1/200 was used as the secondary antibody.  Nuclei are stained in red with DAPI. SNRPD3 is observable in the cell's nuclei.

    See Abreview

  • All lanes : Anti-SNRPD3 antibody (ab111094) at 1 µg/ml

    Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 14 kDa
    Observed band size : 15 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 87 kDa (possible non-specific binding).

    Exposure time : 20 minutes

    SNRPD3 contains a possible methylation site (SwissProt) which may explain its migration at a slightly higher molecular weight than predicted.

    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab111094 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

文献

ab111094 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Permeabilization
Yes - 0.5% Triton X100 in PBS
Fixative
Paraformaldehyde
Username

Dr. Kirk Mcmanus

Verified customer

提交于 Aug 27 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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