概述

  • 产品名称Anti-SNRNP200抗体
    参阅全部 SNRNP200 一抗
  • 描述
    兔多克隆抗体to SNRNP200
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 212-261 (GDEDVYGEVR EEASDDDMEG DEAVVRCTLS ANLVASGELM SSKKKDLHPR) of Human SNRNP200 (NP_054733, O75643).

  • 阳性对照
    • COLO205 lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Constituents: 97% PBS, 2% Sucrose
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab118713 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/200.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 244 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Putative RNA helicase involved in the second step of RNA splicing. May promote one or more conformational changes in the dynamic network of RNA-RNA interactions in the spliceosome. Appears to catalyze an ATP-dependent unwinding of U4/U6 RNA duplices.
  • 组织特异性Widely expressed.
  • 疾病相关Defects in SNRNP200 are the cause of retinitis pigmentosa type 33 (RP33) [MIM:610359]. It is a retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well.
  • 序列相似性Belongs to the helicase family. SKI2 subfamily.
    Contains 2 helicase ATP-binding domains.
    Contains 2 helicase C-terminal domains.
    Contains 2 SEC63 domains.
  • 结构域Composed of two similar domains.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Activating signal cointegrator 1 complex subunit 3-like 1 antibody
    • ASCC3L1 antibody
    • BRR2 antibody
    • BRR2 homolog antibody
    • HELIC2 antibody
    • RP33 antibody
    • SNRNP200 antibody
    • U5 small nuclear ribonucleoprotein 200 kDa helicase antibody
    • U5 snRNP-specific 200 kDa protein antibody
    • U5-200KD antibody
    • U520_HUMAN antibody
    see all

Anti-SNRNP200 antibody 图像

  • Anti-SNRNP200 antibody (ab118713) at 1 µg/ml + COLO205 lysate at 10 µg

    Secondary
    Goat anti-Rabbit IgG-HRP

    Predicted band size : 244 kDa

Anti-SNRNP200 antibody (ab118713)参考文献

This product has been referenced in:
  • Liu YC  et al. Cytoplasmic Viral RNA-Dependent RNA Polymerase Disrupts the Intracellular Splicing Machinery by Entering the Nucleus and Interfering with Prp8. PLoS Pathog 10:e1004199 (2014). WB ; Human . Read more (PubMed: 24968230) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa S3)
Specification HeLa S3
Fixative Formaldehyde
Permeabilization Yes - Triton 0.3%
Blocking step Donkey Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Nov 05 2012

Thank you for your reply. ICC/IF has not been tested, thus, you are eligible to use our testing discount program.

DISCOUNT CODE: xxx
Expiration date: xxx

I am very pleased to hear you would like to accept our offer and test ab...

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Thank you for contacting us.

Yes, you can definitely use our testing discount program to try the antibody in IF.

Could you please let me know what type of IF you would like to use it in: ICC/IF (with cultured cells) or IHC-P (paraf...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"