概述

  • 产品名称Anti-Snf1lk抗体
    参阅全部 Snf1lk 一抗
  • 描述
    兔多克隆抗体to Snf1lk
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Pig, Drosophila melanogaster
  • 免疫原

    Synthetic peptide: MVIMSEFSAD PAGQGQGQQK PLRVGFYDIE RTLGKGNFAV VKLARHRVTK, corresponding to a region within N terminal amino acids 1-50 of Human Snf1lk

  • 阳性对照
    • Jurkat cell lysate, Human stomach tissue

性能

应用

Our Abpromise guarantee covers the use of ab64428 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

靶标

  • 功能Transient role during the earliest stages of myocardial cell differentiation and/or primitive chamber formation and may also be important for the earliest stages of skeletal muscle growth and/or differentiation. Potential role in G2/M cell cycle regulation. Inhibits CREB activity by phosphorylating and repressing the CREB-specific coactivators, CRTC1-3.
  • 序列相似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. AMPK subfamily.
    Contains 1 protein kinase domain.
    Contains 1 UBA domain.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Cytoplasm. Nucleus. Translocates to the cytoplasm on phosphorylation where it binds binding to YWHAZ.
  • Information by UniProt
  • 数据库链接
  • 别名
    • KID2 antibody
    • MSK antibody
    • myocardial SNF1 like kinase antibody
    • QIK antibody
    • Qin-induced kinase antibody
    • Salt inducible kinase 1 antibody
    • Salt-inducible protein kinase 1 antibody
    • Serine threonine protein kinase SNF1 like kinase 1 antibody
    • Serine threonine protein kinase SNF1LK antibody
    • Serine/threonine-protein kinase SIK1 antibody
    • Serine/threonine-protein kinase SNF1-like kinase 1 antibody
    • Serine/threonine-protein kinase SNF1LK antibody
    • SIK-1 antibody
    • SIK1 antibody
    • SIK1_HUMAN antibody
    • SNF1 like kinase antibody
    see all

Anti-Snf1lk antibody 图像

  • Anti-Snf1lk antibody (ab64428) at 1.25 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 85 kDa
    Observed band size : 85 kDa
  • ab64428 at 4-8µg/ml staining surface mucous cells and epithelial cells of fundic gland from human stomach; paraffin embedded; magnification 400X.

Anti-Snf1lk antibody (ab64428)参考文献

This product has been referenced in:
  • Wagschal A  et al. Genome-wide identification of microRNAs regulating cholesterol and triglyceride homeostasis. Nat Med 21:1290-7 (2015). Read more (PubMed: 26501192) »
  • Jagannath A  et al. The CRTC1-SIK1 pathway regulates entrainment of the circadian clock. Cell 154:1100-11 (2013). WB ; Mouse . Read more (PubMed: 23993098) »

See all 2 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (Human HepG2 cells)
Gel Running Conditions Reduced Denaturing
Loading amount 15 µg
Specification Human HepG2 cells
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Dec 05 2016

Application Western blot
Sample Mouse Tissue lysate - whole (Mouse hypothalamus)
Loading amount 5 µg
Specification Mouse hypothalamus
Gel Running Conditions Reduced Denaturing (4-20% Tris-Hepes Gel)
Blocking step Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 24°C
Username

Abcam user community

Verified customer

提交于 Jul 26 2012

DISCOUNT CODES:

Test of the protein ab89693 for inhibition: ********
Test of the antibody ab64428 in Mouse: ********



Expiration date for both codes (extended due to the non-availability): ********


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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"