概述

  • 产品名称Anti-SND1抗体
    参阅全部 SND1 一抗
  • 描述
    兔多克隆抗体to SND1
  • 经测试应用适用于: ICC/IF, IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Cow, Dog, Rhesus monkey, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 700 - 800 of Human SND1.

    (Peptide available as ab71185.)

  • 阳性对照
    • This antibody gave a positive signal in mouse and rat liver tissue lysates, as well as in the following Human Whole Cell Lysates: Ramos; HeLa; Jurkat.

性能

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应用

Our Abpromise guarantee covers the use of ab71186 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 102 kDa).Can be blocked with Human SND1 peptide (ab71185).

靶标

  • 功能Functions as a bridging factor between STAT6 and the basal transcription factor. Plays a role in PIM1 regulation of MYB activity. Functions as a transcriptional coactivator for the Epstein-Barr virus nuclear antigen 2 (EBNA2).
  • 组织特异性Ubiquitously expressed.
  • 序列相似性Contains 4 TNase-like domains.
    Contains 1 Tudor domain.
  • 翻译后修饰Phosphorylated by PIM1 in vitro.
  • 细胞定位Cytoplasm. Nucleus. Melanosome. In IL-4 stimulated cells colocalizes with STAT6 in the nucleus. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 100 kDa coactivator antibody
    • EBNA 2 co activator (100kD) antibody
    • EBNA2 coactivator p100 antibody
    • p100 antibody
    • p100 co-activator antibody
    • p100 coactivator antibody
    • p100 EBNA2 co activator antibody
    • SND 1 antibody
    • Snd1 antibody
    • SND1_HUMAN antibody
    • Staphylococcal nuclease and tudor domain containing 1 antibody
    • Staphylococcal nuclease domain containing 1 antibody
    • Staphylococcal nuclease domain containing protein 1 antibody
    • Staphylococcal nuclease domain-containing protein 1 antibody
    • TDRD11 antibody
    • TRD 11 antibody
    • TRD11 antibody
    • Tudor domain containing protein 11 antibody
    • Tudor domain-containing protein 11 antibody
    see all

Anti-SND1 antibody 图像

  • All lanes : Anti-SND1 antibody (ab71186) at 1 µg/ml

    Lane 1 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 102 kDa
    Observed band size : 120 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 62 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
  • IHC image of SND1 staining in Human Cerebral Cortex FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab71186, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
  • All lanes : Anti-SND1 antibody (ab71186) at 1 µg/ml

    Lane 1 : Liver (Mouse) Tissue Lysate
    Lane 2 : Liver (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 102 kDa
    Observed band size : 120 kDa (why is the actual band size different from the predicted?)


    Exposure time : 8 minutes
  • ICC/IF image of ab71186 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab71186, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa cells at 5µg/ml.

Anti-SND1 antibody (ab71186)参考文献

ab71186 has not yet been referenced specifically in any publications.

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