The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
ELISA titre using peptide based assay 1:62500.
Use a concentration of 1.25 µg/ml. Detects a band of approximately 30,40,56, 80 kDa (predicted molecular weight: 101 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
功能Functions as a bridging factor between STAT6 and the basal transcription factor. Plays a role in PIM1 regulation of MYB activity. Functions as a transcriptional coactivator for the Epstein-Barr virus nuclear antigen 2 (EBNA2).