概述

  • 产品名称Anti-SND1抗体
    参阅全部 SND1 一抗
  • 描述
    兔多克隆抗体to SND1
  • 经测试应用适用于: IP, WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Dog, Chimpanzee, Rhesus monkey, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 850 to the C-terminus of Human SND1.

    (Peptide available as ab71184.)

  • 阳性对照
    • This antibody gave a positive signal in the following Human Whole Cell Lysates: Ramos, HeLa - Hydroxyurea Treated (48hr, 2µM), HeLa - Staurosporine Treated (24hr, 500nM), TE 671, HeLa, JEG-3 and Jurkat.

性能

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应用

Our Abpromise guarantee covers the use of ab65078 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 102 kDa (predicted molecular weight: 102 kDa).
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 1 µg/ml.

靶标

  • 功能Functions as a bridging factor between STAT6 and the basal transcription factor. Plays a role in PIM1 regulation of MYB activity. Functions as a transcriptional coactivator for the Epstein-Barr virus nuclear antigen 2 (EBNA2).
  • 组织特异性Ubiquitously expressed.
  • 序列相似性Contains 4 TNase-like domains.
    Contains 1 Tudor domain.
  • 翻译后修饰Phosphorylated by PIM1 in vitro.
  • 细胞定位Cytoplasm. Nucleus. Melanosome. In IL-4 stimulated cells colocalizes with STAT6 in the nucleus. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 100 kDa coactivator antibody
    • EBNA 2 co activator (100kD) antibody
    • EBNA2 coactivator p100 antibody
    • p100 antibody
    • p100 co-activator antibody
    • p100 coactivator antibody
    • p100 EBNA2 co activator antibody
    • SND 1 antibody
    • Snd1 antibody
    • SND1_HUMAN antibody
    • Staphylococcal nuclease and tudor domain containing 1 antibody
    • Staphylococcal nuclease domain containing 1 antibody
    • Staphylococcal nuclease domain containing protein 1 antibody
    • Staphylococcal nuclease domain-containing protein 1 antibody
    • TDRD11 antibody
    • TRD 11 antibody
    • TRD11 antibody
    • Tudor domain containing protein 11 antibody
    • Tudor domain-containing protein 11 antibody
    see all

Anti-SND1 antibody 图像

  • SND1 was immunoprecipitated using 0.5mg Ramos whole cell extract, 5µg of Rabbit polyclonal to SND1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Ramos whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab65078.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 102kDa: SND1.
  • All lanes : Anti-SND1 antibody (ab65078) at 1 µg/ml

    Lane 1 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 2 : HeLa Whole Cell Lysate - Hydroxyurea Treated (48hr, 2µM)
    Lane 3 : HeLa Whole Cell Lysate - Staurosporine Treated (24hr, 500nM)
    Lane 4 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
    Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 6 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate
    Lane 7 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 102 kDa
    Observed band size : 102 kDa
  • ICC/IF image of ab65078 stained Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65078, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa, HepG2, and MCF-7 cells at 1µg/ml, and in 100% Methanol fixed (5 min) HeLa, Hek293, HepG2, and MCF-7 cells at 1µg/ml.
  • IHC image of ab65078 staining SND1 in Human normal breast formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab65078, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-SND1 antibody (ab65078)参考文献

ab65078 has not yet been referenced specifically in any publications.

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