- 数据表
- 具体的参考文献 (2)
- 实验方案
概述
-
产品名称Anti-SNAPIN抗体
参阅全部 SNAPIN 一抗 -
描述鸡多克隆抗体to SNAPIN
-
经测试应用适用于: WBmore details
-
种属反应性与反应: Mouse, Rat, Human
-
免疫原
Synthetic peptide, corresponding to amino acids 31-136 of Human SNAPIN
-
阳性对照
- Brain tissue
性能
-
形式Liquid
-
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
-
存储溶液Preservative: None
Constituents: PBS -
Concentration information loading... -
纯度Immunogen affinity purified
-
克隆多克隆
-
同种型IgY
-
研究领域
应用
Our Abpromise guarantee covers the use of ab37496 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/2000. Detects a band of approximately 70 kDa for the fusion protein (predicted molecular weight for the native protein: 15 kDa). |
靶标
-
功能May modulate a step between vesicle priming, fusion and calcium-dependent neurotransmitter release by potentiating the interaction of synaptotagmins with the SNAREs and the plasma-membrane-associated protein SNAP25. Its phosphorylation state influences exocytotic protein interactions and may regulate synaptic vesicle exocytosis. May also have a role in the mechanisms of SNARE-mediated membrane fusion in non-neuronal cells.
-
组织特异性Expressed in male germ cells of adult testis (at protein level).
-
序列相似性Belongs to the SNAPIN family.
-
发展阶段Expressed in germ cells of 22-week prenatal testis.
-
细胞定位Cytoplasm. Membrane. Cytoplasmic vesicle membrane. Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cell junction > synapse > synaptosome. Cytoplasm > perinuclear region. May be cytoplasmic and peripheral membrane bound or anchored to the vesicular membrane through an N-terminal signal anchor (By similarity). Co-localizes with NANOS1 and PUM2 in the perinuclear region of germ cells.
- Information by UniProt
-
数据库链接
- Entrez Gene: 23557 Human
- Entrez Gene: 20615 Mouse
- Entrez Gene: 295217 Rat
- Omim: 607007 Human
- SwissProt: O95295 Human
- SwissProt: Q9Z266 Mouse
- SwissProt: P60192 Rat
- Unigene: 32018 Human
see all -
别名
- OTTHUMP00000035157 antibody
- SNAP associated protein antibody
- SNAP-25-binding protein antibody
see all
Anti-SNAPIN antibody 图像
-
Western blot - SNAPIN antibody (ab37496)Anti-SNAPIN antibody (ab37496) at 1/2000 dilution + E. coli derived fusion protein at 2.5 µg
Secondary
Goat anti IgY HRP at 1/1000 dilution
Observed band size : 70 kDa (why is the actual band size different from the predicted?)
Anti-SNAPIN antibody (ab37496)参考文献
This product has been referenced in:
- Zhang Q et al. E3 ubiquitin ligase RNF13 involves spatial learning and assembly of the SNARE complex. Cell Mol Life Sci 70:153-65 (2013). Read more (PubMed: 22890573) »
- Ginter-Matuszewska B et al. The SNARE-associated component SNAPIN binds PUMILIO2 and NANOS1 proteins in human male germ cells. Mol Hum Reprod 15:173-9 (2009). Read more (PubMed: 19168546) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"