概述

  • 产品名称
    Anti-SNAPIN抗体
    参阅全部 SNAPIN 一抗
  • 描述
    鸡多克隆抗体to SNAPIN
  • 宿主
    Chicken
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide, corresponding to amino acids 31-136 of Human SNAPIN

  • 阳性对照
    • Brain tissue

性能

应用

Our Abpromise guarantee covers the use of ab37496 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000. Detects a band of approximately 70 kDa for the fusion protein (predicted molecular weight for the native protein: 15 kDa).

靶标

  • 功能
    May modulate a step between vesicle priming, fusion and calcium-dependent neurotransmitter release by potentiating the interaction of synaptotagmins with the SNAREs and the plasma-membrane-associated protein SNAP25. Its phosphorylation state influences exocytotic protein interactions and may regulate synaptic vesicle exocytosis. May also have a role in the mechanisms of SNARE-mediated membrane fusion in non-neuronal cells.
  • 组织特异性
    Expressed in male germ cells of adult testis (at protein level).
  • 序列相似性
    Belongs to the SNAPIN family.
  • 发展阶段
    Expressed in germ cells of 22-week prenatal testis.
  • 细胞定位
    Cytoplasm. Membrane. Cytoplasmic vesicle membrane. Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cell junction > synapse > synaptosome. Cytoplasm > perinuclear region. May be cytoplasmic and peripheral membrane bound or anchored to the vesicular membrane through an N-terminal signal anchor (By similarity). Co-localizes with NANOS1 and PUM2 in the perinuclear region of germ cells.
  • Information by UniProt
  • 数据库链接
    see all
  • 别名
    • OTTHUMP00000035157 antibody
    • SNAP associated protein antibody
    • SNAP-25-binding protein antibody
    • SNAP-associated protein antibody
    • SNAP25BP antibody
    • SNAPAP antibody
    • Snapin antibody
    • SNAPN_HUMAN antibody
    • SNARE associated protein snapin antibody
    • SNARE-associated protein Snapin antibody
    • Synaptosomal associated protein 25 binding protein antibody
    • Synaptosomal-associated protein 25-binding protein antibody
    see all

图片

  • Western blot - SNAPIN antibody (ab37496)
    Western blot - SNAPIN antibody (ab37496)
    Anti-SNAPIN antibody (ab37496) at 1/2000 dilution + E. coli derived fusion protein at 2.5 µg

    Secondary
    Goat anti IgY HRP at 1/1000 dilution

    Observed band size: 70 kDa (why is the actual band size different from the predicted?)



    Band size larger than expected as a fusion protein was used as positive control.

实验方案

文献

This product has been referenced in:
  • Zhang Q  et al. E3 ubiquitin ligase RNF13 involves spatial learning and assembly of the SNARE complex. Cell Mol Life Sci 70:153-65 (2013). Read more (PubMed: 22890573) »
  • Blandin G  et al. A human skeletal muscle interactome centered on proteins involved in muscular dystrophies: LGMD interactome. Skelet Muscle 3:3 (2013). WB . Read more (PubMed: 23414517) »

See all 3 Publications for this product

发表研究结果有使用 ab37496？请让我们知道，以便我们可以引用本数据表中的参考文章。

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