Anti-SNAP45抗体(ab23467)

概述

  • 产品名称Anti-SNAP45抗体
    参阅全部 SNAP45 一抗
  • 描述
    兔多克隆抗体to SNAP45
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide: STEEDFAVDF EKIYKYLSSV SRSGRSPELS AAESAVVLDL LMSLPEELPL, corresponding to internal sequence amino acids 197-246 of Human SNAP45

  • 阳性对照
    • Jurkat cell lysate for WB. Paraffin embedded human kidney cells and epithelial cells of renal tubule for IHC.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab23467 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.4 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 36 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/62500.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能Part of the SNAPc complex required for the transcription of both RNA polymerase II and III small-nuclear RNA genes. Binds to the proximal sequence element (PSE), a non-TATA-box basal promoter element common to these 2 types of genes. Recruits TBP and BRF2 to the U6 snRNA TATA box.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Proximal sequence element binding transcription factor delta subunit antibody
    • Proximal sequence element binding transcription factor subunit delta antibody
    • Proximal sequence element-binding transcription factor subunit delta antibody
    • PSE binding factor delta subunit antibody
    • PSE binding factor subunit delta antibody
    • PSE-binding factor subunit delta antibody
    • PTF delta subunit antibody
    • PTF subunit delta antibody
    • PTFdelta antibody
    • Small nuclear RNA activating complex polypeptide 2 antibody
    • Small nuclear RNA-activating complex polypeptide 2 antibody
    • SNAP45 antibody
    • SNAPC 2 antibody
    • SNAPc 45 kDa subunit antibody
    • SNAPc subunit 2 antibody
    • Snapc2 antibody
    • SNPC2_HUMAN antibody
    • snRNA activating protein complex 2 antibody
    • snRNA activating protein complex 45 kDa subunit antibody
    • snRNA activating protein complex subunit 2 antibody
    • snRNA-activating protein complex 45 kDa subunit antibody
    • snRNA-activating protein complex subunit 2 antibody
    see all

Anti-SNAP45 antibody 图像

  • Anti-SNAP45 antibody (ab23467) at 0.4 µg/ml + Jurkat cell lysate 20ug.

    Secondary
    HRP conjugated anti-rabbit IgG diluted at 1/50,000-1/100,000.

    Predicted band size : 36 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling SNAP45 with ab23467 at a concentration of 4-8µg/ml. Cells with positive labels (arrows) are Epithelial cells of renal tubule.

Anti-SNAP45 antibody (ab23467)参考文献

ab23467 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Embryo)
Specification Embryo
Fixative Paraformaldehyde
Permeabilization Yes - Triton-X-1oo
Blocking step Donkey serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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提交于 Jul 02 2012

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