SNAP25 overexpression 293T裂解物(whole cell) (ab94226)
概述
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产品名称
SNAP25 overexpression 293T裂解物(whole cell)
参阅全部 SNAP25 细胞裂解液 -
常规说明
ab94226 is a 293T cell transfected lysate in which Human SNAP25 has been transiently over-expressed using a pCMV-SNAP25 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes.
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经测试应用
适用于: WBmore details
性能
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Mycoplasma free
Yes -
形式
Liquid -
存放说明
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
存储溶液
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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研究领域
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背景
Function: t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF. Tissue specificity: Neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum. Similarity: Belongs to the SNAP-25 family. Contains 2 t-SNARE coiled-coil homology domains. PTM: Palmitoylated. Cys-85 appears to be the main site, and palmitoylation is required for membrane association.
相关产品
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Corresponding Antibody
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab94226于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use at an assay dependent dilution.
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说明 |
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WB
Use at an assay dependent dilution. |
图片
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ab94226 at 15μg/lane on an SDS-PAGE gel
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All lanes : Anti-SNAP25 antibody [4A3] (ab66066) at 1/500 dilution
Lane 1 :SNAP25 overexpression 293T lysate (whole cell) (ab94226)
Lane 2 : 293T Non Transfected Lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab94226 尚未被引用在任何文献中。