常规说明This product should be stored undiluted.
Storage in frost free freezers is not recommended.
Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of despatch.
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用说明ELISA: Use at an assay dependent dilution.
IHC-P: 1/2000 - 1/5000. This product does not require protein digestion pre-treatment or antigen retrieval using heat treatment prior to staining of paraffin sections.
WB: 1/500 - 1/5000. Detects a band of approximately 26-27 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
功能t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF.
组织特异性Neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum.
序列相似性Belongs to the SNAP-25 family. Contains 2 t-SNARE coiled-coil homology domains.
翻译后修饰Palmitoylated. Cys-85 appears to be the main site, and palmitoylation is required for membrane association.
细胞定位Cytoplasm > perinuclear region. Cell membrane. Cell junction > synapse > synaptosome. Membrane association requires palmitoylation. Expressed throughout cytoplasm, concentrating at the perinuclear region.