RabMAb

Anti-SNAP25抗体[EPR3275] (ab109105)

概述

  • 产品名称Anti-SNAP25抗体[EPR3275]
    参阅全部 SNAP25 一抗
  • 描述
    兔单克隆抗体[EPR3275] to SNAP25
  • 经测试应用适用于: WB, IP, ICC/IFmore details
    不适用于: Flow Cyt or IHC-P
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human SNAP25.

  • 阳性对照
    • Human fetal brain, SH SY5Y, and Neuro 2a lysates; SH SY5Y cells.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

应用

Our Abpromise guarantee covers the use of ab109105 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 23 kDa.
IP 1/10 - 1/100.
ICC/IF 1/100 - 1/250.
  • 应用说明Is unsuitable for Flow Cyt or IHC-P.
  • 靶标

    • 功能t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF.
    • 组织特异性Neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum.
    • 序列相似性Belongs to the SNAP-25 family.
      Contains 2 t-SNARE coiled-coil homology domains.
    • 翻译后修饰Palmitoylated. Cys-85 appears to be the main site, and palmitoylation is required for membrane association.
    • 细胞定位Cytoplasm > perinuclear region. Cell membrane. Cell junction > synapse > synaptosome. Membrane association requires palmitoylation. Expressed throughout cytoplasm, concentrating at the perinuclear region.
    • Information by UniProt
    • 数据库链接
    • 别名
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      • RIC 4 antibody
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      • SNAP 25 antibody
      • SNAP antibody
      • SNAP-25 antibody
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      • SNAP25 antibody
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      • Synaptosomal-associated 25 kDa protein antibody
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      see all

    Anti-SNAP25 antibody [EPR3275] 图像

    • All lanes : Anti-SNAP25 antibody [EPR3275] (ab109105) at 1/1000 dilution

      Lane 1 : Human fetal brain lysate
      Lane 2 : SH SY5Y lysate
      Lane 3 : Neuro 2a lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 23 kDa
    • Immunofluorescent staining of SNAP25 in SH SY5Y cells using ab109105 at 1/100.

    Anti-SNAP25 antibody [EPR3275] (ab109105)参考文献

    This product has been referenced in:
    • Cheng YC  et al. Knocking down of heat-shock protein 27 directs differentiation of functional glutamatergic neurons from placenta-derived multipotent cells. Sci Rep 6:30314 (2016). ICC/IF ; Human . Read more (PubMed: 27444754) »

    See 1 Publication for this product

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    Thank you for contacting us.

    The peptide maps between amino acids 180 - 206 of human SNAP-25, at the C-terminus (UniProt: P60880).

    Please let me know if you have any questions.


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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"