The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Detects a band of approximately 25 kDa (predicted molecular weight: 23 kDa).
1/100 - 1/250. Antigen retrieval is recommended.
1/100 - 1/250.
应用说明Is unsuitable for Flow Cyt or IP.
功能t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF.
组织特异性Neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum.
序列相似性Belongs to the SNAP-25 family. Contains 2 t-SNARE coiled-coil homology domains.
翻译后修饰Palmitoylated. Cys-85 appears to be the main site, and palmitoylation is required for membrane association.
细胞定位Cytoplasm > perinuclear region. Cell membrane. Cell junction > synapse > synaptosome. Membrane association requires palmitoylation. Expressed throughout cytoplasm, concentrating at the perinuclear region.
ab108990 showing negative staining in Skeletal muscle tissue.
Anti-SNAP25 antibody [EP3274] (ab108990)参考文献
This product has been referenced in:
Lue LF et al. TREM2 Protein Expression Changes Correlate with Alzheimer's Disease Neurodegenerative Pathologies in Post-Mortem Temporal Cortices. Brain Pathol25:469-80 (2015).
Read more (PubMed: 25186950) »