Anti-SNAP25抗体(ab66066)

概述

  • 产品名称Anti-SNAP25抗体
    参阅全部 SNAP25 一抗
  • 描述
    小鼠单克隆抗体to SNAP25
  • 经测试应用适用于: ELISA, WB, ICC/IF, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Human, Zebrafish
    预测可用于: Rat, Chicken, Dog, Xenopus laevis, Cynomolgus Monkey, Opossum, Orangutan
  • 免疫原

    Recombinant tagged fragment: RMLQLVEESK DAGIRTLVML DEQGEQLDRV EEGMNHINQD MKEAEKNLKD LGKCCGLFIC PCNKLKSSDA YKKAWGNNQD GVVASQPARV VDEREQMAIS, corresponding to amino acids 31-130 of Human SNAP25

  • 阳性对照
    • SNAP25 transfected 293T cell lysate. This antibody gave a positive result in IF in the following Formaldehyde fixed cell line: SKNSH.

性能

应用

Our Abpromise guarantee covers the use of ab66066 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 23 kDa).
ICC/IF Use a concentration of 5 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF.
  • 组织特异性Neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum.
  • 序列相似性Belongs to the SNAP-25 family.
    Contains 2 t-SNARE coiled-coil homology domains.
  • 翻译后修饰Palmitoylated. Cys-85 appears to be the main site, and palmitoylation is required for membrane association.
  • 细胞定位Cytoplasm > perinuclear region. Cell membrane. Cell junction > synapse > synaptosome. Membrane association requires palmitoylation. Expressed throughout cytoplasm, concentrating at the perinuclear region.
  • Information by UniProt
  • 数据库链接
  • 别名
    • bA416N4.2 antibody
    • Bdr antibody
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    • dJ1068F16.2 antibody
    • FLJ23079 antibody
    • HGNC:11132 antibody
    • MGC105414 antibody
    • MGC139754 antibody
    • Resistance to inhibitors of cholinesterase 4 homolog antibody
    • RIC 4 antibody
    • RIC4 antibody
    • SEC 9 antibody
    • SEC9 antibody
    • SNAP 25 antibody
    • SNAP antibody
    • SNAP-25 antibody
    • SNAP-25B antibody
    • SNAP25 antibody
    • SNP 25 antibody
    • SNP25 antibody
    • SNP25_HUMAN antibody
    • sp antibody
    • SUP antibody
    • Super protein antibody
    • Synaptosomal associated 25 kDa protein antibody
    • Synaptosomal associated protein antibody
    • Synaptosomal associated protein 25 antibody
    • Synaptosomal associated protein 25kDa antibody
    • Synaptosomal-associated 25 kDa protein antibody
    • Synaptosomal-associated protein 25 antibody
    • Synaptosomal-associated protein, 25-KD antibody
    see all

Anti-SNAP25 antibody 图像

  • All lanes : Anti-SNAP25 antibody (ab66066) at 1 µg/ml

    Lane 1 : SNAP25 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 23 kDa
    Observed band size : 27 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-SNAP25 antibody (ab66066) at 1 µg/ml

    Lane 1 : Marker
    Lane 2 : Zebrafish brain homogenate at 20 µg
    Lane 3 : SH-SY5Y (Human neuroblastoma cell line) whole cell lysate at 20 µg
    Lane 4 : Mouse brain homogenate at 20 µg

    Secondary
    Goat polyclonal to Mouse IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 23 kDa
    Observed band size : 26 kDa (why is the actual band size different from the predicted?)


    Exposure time : 2 minutes
  • Overlay histogram showing SH-SY5Y cells stained with ab66066 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab66066, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • ab66066 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab66066 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-SNAP25 antibody (ab66066)参考文献

ab66066 has not yet been referenced specifically in any publications.

Product Wall

Thank you for providing the additional information. I see your earlier e-mail in our records. We have 15 SNAP25 antibodies in our catalogue. Some are polyclonals raised against full-length SNAP25, and since these will potentially recognize multi...

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