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Our Abpromise guarantee covers the use of ab5666 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use a concentration of 5 µg/ml.|
Immunofluorescence staining of SNAP-25 in cultured rat hippocampal cells with ab5666 yields a pattern consistent with cytoplasmic and plasma membrane staining.
|WB||Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 25 kDa.Can be blocked with SNAP25 peptide (ab5843).|
|ICC||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|Flow Cyt||Use 3-5µg for 106 cells.|
Flow cytometry analysis of Neuro-2a cells using ab5666.Cells were fixed with 70% ethanol for 10 minutes,permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature.Cells were labeled with SNAP25 (red histogram) or with rabbit isotype control (pink histogram) at 3-5 ug/million cells in 2.5% BSA.After incubation at room temperature for 2 hours,the cells were labeled with Alexa Fluor® 488 Goat Anti-Rabbit Secondary Antibody at a dilution of 1:400 for 30 minutes at room temperature.The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.
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