Anti-SNAP25抗体(ab41726)

概述

  • 产品名称Anti-SNAP25抗体
    参阅全部 SNAP25 一抗
  • 描述
    兔多克隆抗体to SNAP25
  • 经测试应用适用于: IP, ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human, Zebrafish
    预测可用于: Chicken, Cow, Drosophila melanogaster
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human SNAP25.

    (Peptide available as ab41725.)

  • 阳性对照
    • This antibody gave a positive signal in the following lysates: Spinal Cord (Mouse) Tissue Lysate, Spinal Cord (Rat) Tissue lysate, Hippocampus (Mouse) Tissue Lysate.

性能

应用

Our Abpromise guarantee covers the use of ab41726 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 23 kDa).Can be blocked with Human SNAP25 peptide (ab41725).

靶标

  • 功能t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF.
  • 组织特异性Neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum.
  • 序列相似性Belongs to the SNAP-25 family.
    Contains 2 t-SNARE coiled-coil homology domains.
  • 翻译后修饰Palmitoylated. Cys-85 appears to be the main site, and palmitoylation is required for membrane association.
  • 细胞定位Cytoplasm > perinuclear region. Cell membrane. Cell junction > synapse > synaptosome. Membrane association requires palmitoylation. Expressed throughout cytoplasm, concentrating at the perinuclear region.
  • Information by UniProt
  • 数据库链接
  • 别名
    • bA416N4.2 antibody
    • Bdr antibody
    • CMS18 antibody
    • dJ1068F16.2 antibody
    • FLJ23079 antibody
    • HGNC:11132 antibody
    • MGC105414 antibody
    • MGC139754 antibody
    • Resistance to inhibitors of cholinesterase 4 homolog antibody
    • RIC 4 antibody
    • RIC4 antibody
    • SEC 9 antibody
    • SEC9 antibody
    • SNAP 25 antibody
    • SNAP antibody
    • SNAP-25 antibody
    • SNAP-25B antibody
    • SNAP25 antibody
    • SNP 25 antibody
    • SNP25 antibody
    • SNP25_HUMAN antibody
    • sp antibody
    • SUP antibody
    • Super protein antibody
    • Synaptosomal associated 25 kDa protein antibody
    • Synaptosomal associated protein antibody
    • Synaptosomal associated protein 25 antibody
    • Synaptosomal associated protein 25kDa antibody
    • Synaptosomal-associated 25 kDa protein antibody
    • Synaptosomal-associated protein 25 antibody
    • Synaptosomal-associated protein, 25-KD antibody
    see all

Anti-SNAP25 antibody 图像

  • All lanes : Anti-SNAP25 antibody (ab41726) at 1 µg/ml

    Lane 1 : Spinal Cord (Mouse) Tissue Lysate
    Lane 2 : Spinal Cord (Rat) Tissue lysate
    Lane 3 : Mouse brain tissue lysate - total protein (0 days) (ab7188)

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 23 kDa
    Observed band size : 26 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab41726 stained human SH-SY5Y cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab41726, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

  • All lanes : Anti-SNAP25 antibody (ab41726) at 1 µg/ml

    Lane 1 : Marker
    Lane 2 : Zebrafish brain homogenate at 20 µg
    Lane 3 : SH-SY5Y (Human neuroblastoma cell line) whole cell lysate at 20 µg
    Lane 4 : Mouse brain homogenate at 20 µg

    Secondary
    Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 23 kDa
    Observed band size : 26 kDa (why is the actual band size different from the predicted?)
  • SNAP25 was immunoprecipitated using 0.5mg Rat Spinal Cord tissue lysate, 5µg of Rabbit polyclonal to SNAP25 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Rat Spinal Cord tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab41726.
    Secondary: Clean-Blot IP Detection Reagent (HRP) at 1/500 dilution.
    Band: 26kDa; SNAP25; non specific bands - 55 and 98kDa: We are unsure as to the identity of this extra band.

Anti-SNAP25 antibody (ab41726)参考文献

ab41726 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 40 µg
Gel Running Conditions Non-reduced Denaturing (Extra PAGE ONE 5-20%)
Sample Chicken Tissue lysate - whole (Brain and DRG)
Specification Brain and DRG
Blocking step Blocking one as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 24°C
Username

Abcam user community

Verified customer

提交于 Feb 09 2015

Thank you for providing the additional information. I see your earlier e-mail in our records. We have 15 SNAP25 antibodies in our catalogue. Some are polyclonals raised against full-length SNAP25, and since these will potentially recognize multi...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Brain sections)
Specification Brain sections
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Username

Dr. Sophie Pezet

Verified customer

提交于 Mar 17 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"