概述

  • 产品名称
  • 描述
    山羊多克隆抗体to SNAP25
  • 特异性
    ab31281 recognises SNAP25.
  • 经测试应用
    适用于: IHC-P, WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Dog
  • 免疫原

    Synthetic peptide corresponding to Human SNAP25 aa 193-206 (C terminal).
    Sequence:

    C-DEANQRATKMLGSG

  • 阳性对照
    • WB: Human brain (cerebellum) and mouse brain tissue lysates. ICC/IF: SKNSH cells. IHC-P: Human cerebral cortex tissue.

性能

应用

Our Abpromise guarantee covers the use of ab31281 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 2 - 4 µg/ml.
WB Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 23 kDa).
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 功能
    t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF.
  • 组织特异性
    Neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum.
  • 序列相似性
    Belongs to the SNAP-25 family.
    Contains 2 t-SNARE coiled-coil homology domains.
  • 翻译后修饰
    Palmitoylated. Cys-85 appears to be the main site, and palmitoylation is required for membrane association.
  • 细胞定位
    Cytoplasm > perinuclear region. Cell membrane. Cell junction > synapse > synaptosome. Membrane association requires palmitoylation. Expressed throughout cytoplasm, concentrating at the perinuclear region.
  • Information by UniProt
  • 数据库链接
  • 别名
    • bA416N4.2 antibody
    • Bdr antibody
    • CMS18 antibody
    • dJ1068F16.2 antibody
    • FLJ23079 antibody
    • HGNC:11132 antibody
    • MGC105414 antibody
    • MGC139754 antibody
    • Resistance to inhibitors of cholinesterase 4 homolog antibody
    • RIC 4 antibody
    • RIC4 antibody
    • SEC 9 antibody
    • SEC9 antibody
    • SNAP 25 antibody
    • SNAP antibody
    • SNAP-25 antibody
    • SNAP-25B antibody
    • SNAP25 antibody
    • SNP 25 antibody
    • SNP25 antibody
    • SNP25_HUMAN antibody
    • sp antibody
    • SUP antibody
    • Super protein antibody
    • Synaptosomal associated 25 kDa protein antibody
    • Synaptosomal associated protein antibody
    • Synaptosomal associated protein 25 antibody
    • Synaptosomal associated protein 25kDa antibody
    • Synaptosomal-associated 25 kDa protein antibody
    • Synaptosomal-associated protein 25 antibody
    • Synaptosomal-associated protein, 25-KD antibody
    see all

Anti-SNAP25 antibody 图像

  • ab31281 (1µg/ml) staining of SNAP25 in paraffin embedded Human Cerebellum. Microwaved antigen retrieval with citrate buffer Ph 6, HRP-staining.

     

  • ab31281 negative control showing staining of paraffin embedded Human Cerebellum, with no primary antibody.

  • All lanes : Anti-SNAP25 antibody (ab31281) at 0.03 µg/ml

    Lane 1 : Human brain (cerebellum) tissue lysate in RIPA buffer
    Lane 2 : Mouse brain tissue lysate in RIPA buffer

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique

    Predicted band size : 23 kDa

    Incubated with the primary antibody for 1 hour.

  • ICC/IF image of ab31281 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab31281 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 donkey anti- goat (ab96931) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab31281 at 2.5µg/ml staining SNAP25 in human cerebral cortex tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in steam with citrate buffer (pH 6.0). The AP-staining procedure was used for detection.

Anti-SNAP25 antibody (ab31281)参考文献

This product has been referenced in:
  • Enriquez-Algeciras M  et al. Deimination restores inner retinal visual function in murine demyelinating disease. J Clin Invest N/A:N/A (2013). ELISA ; Rat . Read more (PubMed: 23281397) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Loading amount
10 µg
Specification
HeLa
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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提交于 Oct 18 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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