Anti-SNAP25抗体(ab31281)
Key features and details
- Goat polyclonal to SNAP25
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-SNAP25抗体
参阅全部 SNAP25 一抗 -
描述
山羊多克隆抗体to SNAP25 -
宿主
Goat -
特异性
This antibody is expected to recognise both reported isoforms NP_003072.2 (SNAP25A) and NP_570824.1 (SNAP25B). Reported variants represent identical protein: NP_001309831.1, NP_003072.2 Reported variants represent identical protein: NP_001309834.1, NP_001309832.1, NP_001309833.1, NP_001309839.1, NP_001309836.1, NP_570824.1, NP_001309838.1, NP_001309835.1, NP_001309837.1 -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Dog -
免疫原
Synthetic peptide corresponding to Human SNAP25 aa 193-206 (C terminal).
Sequence:C-DEANQRATKMLGSG
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阳性对照
- WB: Human, mouse and rat brain tissue lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab31281于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
Use a concentration of 1 - 2 µg/ml.
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WB | (1) |
Use a concentration of 0.005 - 0.01 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 23 kDa).
1 hour primary incubation is recommended for this product. |
说明 |
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IHC-P
Use a concentration of 1 - 2 µg/ml. |
WB
Use a concentration of 0.005 - 0.01 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 23 kDa). 1 hour primary incubation is recommended for this product. |
靶标
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功能
t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF. -
组织特异性
Neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum. -
序列相似性
Belongs to the SNAP-25 family.
Contains 2 t-SNARE coiled-coil homology domains. -
翻译后修饰
Palmitoylated. Cys-85 appears to be the main site, and palmitoylation is required for membrane association. -
细胞定位
Cytoplasm > perinuclear region. Cell membrane. Cell junction > synapse > synaptosome. Membrane association requires palmitoylation. Expressed throughout cytoplasm, concentrating at the perinuclear region. - Information by UniProt
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数据库链接
- Entrez Gene: 6616 Human
- Entrez Gene: 20614 Mouse
- Entrez Gene: 25012 Rat
- Omim: 600322 Human
- SwissProt: P60880 Human
- SwissProt: P60879 Mouse
- SwissProt: P60881 Rat
- Unigene: 167317 Human
see all -
别名
- bA416N4.2 antibody
- Bdr antibody
- CMS18 antibody
see all
图片
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All lanes : Anti-SNAP25 antibody (ab31281) at 0.005 µg/ml
Lane 1 : Human Brain (Cerebellum) tissue lysate
Lanes 2-3 : Mouse Brain tissue lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 23 kDaLysates in RIPA buffer. Detected by chemiluminescence.
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ab31281 (1µg/ml) staining of SNAP25 in paraffin embedded Human Cerebellum. Microwaved antigen retrieval with citrate buffer Ph 6, HRP-staining.
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ab31281 negative control showing staining of paraffin embedded Human Cerebellum, with no primary antibody.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab31281 被引用在 3 文献中.
- Liang D et al. Sinoatrial node pacemaker cells share dominant biological properties with glutamatergic neurons. Protein Cell 12:545-556 (2021). PubMed: 33548033
- Mizuta H et al. Additive Effects of L-Ornithine on Preferences to Basic Taste Solutions in Mice. Nutrients 13:N/A (2021). PubMed: 34836006
- Enriquez-Algeciras M et al. Deimination restores inner retinal visual function in murine demyelinating disease. J Clin Invest N/A:N/A (2013). ELISA ; Rat . PubMed: 23281397