概述

  • 产品名称
    Anti-SNAIL + SLUG抗体
    参阅全部 SNAIL + SLUG 一抗
  • 描述
    兔多克隆抗体to SNAIL + SLUG
  • 经测试应用
    适用于: IHC-Fr, WB, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide within Human SNAIL+SLUG aa 236-264. The exact sequence is proprietary.
    Database link: O95863

  • 阳性对照
    • WB: HeLa, A431, MCF7, K563, SW620 and HepG2 cell lysates; mouse lung, mouse heart and rat liver tissue lysates. IHC-P: Human kidney, human colon carcinoma, mouse testis and rat brain tissues.

性能

应用

Our Abpromise guarantee covers the use of ab180714 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr 1/100.
WB 1/500 - 1/2000. Predicted molecular weight: 29 kDa.
ICC/IF 1/50 - 1/200.
IHC-P 1/50 - 1/200.

靶标

  • 相关性
    Function: SNAIL is involved in the epithelial to mesenchymal transition (EMT) and formation and maintenance of embryonic mesoderm (By similarity). Binds to 3 E-boxes of the E-cadherin gene promoter and represses its transcription. SLUG is a transcriptional repressor, involved in the generation and migration of neural crest cells. PTM: SNAIL is phosphorylated by GSK3B. Once phosphorylated, it becomes a target for BTRC ubiquitination. Ubiquitinated on Lys-98, Lys-137 and Lys-146 by FBXL14 and BTRC leading to degradation. BTRC-triggered ubiquitination requires previous GSK3B-mediated SNAI1 phosphorylation. Similarity: Both SNAIL and SLUG belong to the snail C2H2-type zinc-finger protein family. Tissue specificity: SNAIL is expressed in a variety of tissues with the highest expression in kidney. Expressed in mesenchymal and epithelial cell lines. SLUG is expressed in placenta and adult heart, pancreas, liver, kidney and skeletal muscle.
  • 细胞定位
    Slug is generally nuclear, while Snail is known to be both cytoplasmic and nuclear. Once phosphorylated (probably on Ser-107, Ser-111, Ser-115 and Ser-119) snail is exported from the nucleus to the cytoplasm where subsequent phosphorylation of the destruction motif and ubiquitination involving BTRC occurs.
  • 数据库链接
  • 别名
    • dJ710H13.1 antibody
    • MGC10182 antibody
    • Neural crest transcription factor Slug antibody
    • Protein sna antibody
    • Protein snail homolog 1 antibody
    • Protein snail homolog 2 antibody
    • Protein snail homolog antibody
    • Slug homolog zinc finger protein antibody
    • Slug zinc finger protein antibody
    • SLUGH antibody
    • SLUGH 1 antibody
    • SLUGH1 antibody
    • SLUGH2 antibody
    • SNA antibody
    • Sna protein antibody
    • SNAH antibody
    • SNAI 2 antibody
    • snai1 antibody
    • SNAI1_HUMAN antibody
    • Snai2 antibody
    • SNAI2_HUMAN antibody
    • Snail 2 antibody
    • Snail homolog 1 (Drosophila) antibody
    • Snail homolog 2 antibody
    • Snail2 antibody
    • WS 2D antibody
    • WS2D antibody
    • Zinc finger protein SLUG antibody
    • Zinc finger protein SNAI1 antibody
    • Zinc finger protein SNAI2 antibody
    see all

图片

  • Anti-SNAIL + SLUG antibody (ab180714) at 1/500 dilution + Mouse heart tissue extracts

    Predicted band size : 29 kDa
  • All lanes : Anti-SNAIL + SLUG antibody (ab180714)

    Lane 1 : HeLa cell lysate
    Lane 2 : A431 cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : Mouse lung tissue lysate
    Lane 5 : Rat liver tissue lysate


    Predicted band size : 29 kDa
  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling SNAI1+SLUG with ab180714 at 1/50 dilution.

  • ab180714 staining SNAIL in human MCF10A cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 3.7% PFA in PBS for 15 minutes and blocked with 0.1% Milk + 0.1% Tween in PBS for 30 minutes at 25°C. Samples were incubated with primary antibody (1/1000 in PBS + 0.1% Tween) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue labeling SNAIL + SLUG with ab180714 at 1/100 dilution.



  • Predicted band size : 29 kDa

    Western blot analysis of extracts of various cell lines using ab180714 at the dilution 1/500.

  • ab180714 staining SNAIL in Mouse kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde; antigen retrieval was by heat mediation in a basic buffer (TRIS+EDTA, pH9). Samples were incubated with primary antibody (1/500 in antibody diluent) for 1 hour. An undiluted HRP-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling SNAIL with ab180714. Tissue was fixed with formaldehyde and blocked with 5% BSA for 20 min at room temperature; antigen retrieval was by heat mediation with EDTA buffer (pH 9.0). Samples were incubated with primary antibody (1/100) for 12 hours at 4°C. A Biotin-conjugated mouse anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Magnification: 200X.

  • Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue labeling SNAIL +SLUG with ab180714 at 1/100 dilution.



  • Predicted band size : 29 kDa

    Lane 1: Snail recombinant 0.1 ug (55 kDa)

    Lane 2: Slug recombinant 0.1 ug  (56 kDa)

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling SNAIL with ab180714. Tissue was fixed with formaldehyde and blocked with 5% BSA for 20 min at room temperature; antigen retrieval was by heat mediation with EDTA buffer (pH 9.0). Samples were incubated with primary antibody (1/100) for 12 hours at 4°C. A Biotin-conjugated mouse anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Magnification: 400X.

  • ab180714 staining SNAIL in mouse embryonic heart tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 1% BSA for 30 minutes at room temperature. Samples were incubated with primary antibody (1/100 in blocking buffer) for 16 hours at 4°C. An undiluted Alexa Fluor® 488-conjugated donkey anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

文献

This product has been referenced in:
  • Yang S  et al. STC2 promotes head and neck squamous cell carcinoma metastasis through modulating the PI3K/AKT/Snail signaling. Oncotarget 8:5976-5991 (2017). WB ; Human . Read more (PubMed: 27863406) »
  • Jaca A  et al. The role of miRNA-21 and epithelial mesenchymal transition (EMT) process in colorectal cancer. J Clin Pathol 70:331-356 (2017). Human . Read more (PubMed: 27672217) »

See all 31 Publications for this product

客户评价及客户问答

Filter by Application

Filter by Species

Filter by Ratings

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Basic buffer (TRIS+EDTA, pH9)
Permeabilization
No
Specification
Kidney
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Oct 12 2015

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% TritonX100 in PBS
Specification
HeLa
Fixative
Paraformaldehyde
Username

Dr. Kirk Mcmanus

Verified customer

提交于 Aug 07 2015

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 0.1% · Temperature: 25°C
Sample
Human Cell (MCF10A)
Specification
MCF10A
Permeabilization
No
Fixative
3,7% PFA in PBS, 15mins
Username

Abcam user community

Verified customer

提交于 Sep 29 2014

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (4-20%)
Sample
Human Cell lysate - whole cell (MCF10A)
Specification
MCF10A
Treatment
TGFb2 2ng/ml for 48h
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Sep 26 2014

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (4-20%)
Sample
Mouse Cell lysate - whole cell (mouse pre-iPS neural cells)
Specification
mouse pre-iPS neural cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 Aug 21 2014

Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
Sample
Mouse Tissue sections (embryonic heart)
Specification
embryonic heart
Permeabilization
No
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Jun 03 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册