Anti-SNAIL + SLUG抗体[20C8] (ab78105)

概述

  • 产品名称Anti-SNAIL + SLUG抗体[20C8]
    参阅全部 SNAIL + SLUG 一抗
  • 描述
    小鼠单克隆抗体[20C8] to SNAIL + SLUG
  • 特异性This antibody detects endogonous proteins in the following cell lines: MDAMB-453; MDAMB-435; MDAMB-231; A375.
  • 经测试应用适用于: Sandwich ELISA, ELISA, WB, IP, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length protein (Human)

性能

应用

Our Abpromise guarantee covers the use of ab78105 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use a concentration of 1 µg/ml. For sandwich ELISA, use this antibody as Capture at 1 µg/ml with Rabbit polyclonal to SNAIL - ChIP Grade (ab85931) as Detection.
ELISA Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Predicted molecular weight: 29 kDa.
IP Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.

靶标

  • 相关性Function: SNAIL is involved in the epithelial to mesenchymal transition (EMT) and formation and maintenance of embryonic mesoderm (By similarity). Binds to 3 E-boxes of the E-cadherin gene promoter and represses its transcription. SLUG is a transcriptional repressor, involved in the generation and migration of neural crest cells. PTM: SNAIL is phosphorylated by GSK3B. Once phosphorylated, it becomes a target for BTRC ubiquitination. Ubiquitinated on Lys-98, Lys-137 and Lys-146 by FBXL14 and BTRC leading to degradation. BTRC-triggered ubiquitination requires previous GSK3B-mediated SNAI1 phosphorylation. Similarity: Both SNAIL and SLUG belong to the snail C2H2-type zinc-finger protein family. Tissue specificity: SNAIL is expressed in a variety of tissues with the highest expression in kidney. Expressed in mesenchymal and epithelial cell lines. SLUG is expressed in placenta and adult heart, pancreas, liver, kidney and skeletal muscle.
  • 细胞定位Slug is generally nuclear, while Snail is known to be both cytoplasmic and nuclear. Once phosphorylated (probably on Ser-107, Ser-111, Ser-115 and Ser-119) snail is exported from the nucleus to the cytoplasm where subsequent phosphorylation of the destruction motif and ubiquitination involving BTRC occurs.
  • 数据库链接
  • 别名
    • dJ710H13.1 antibody
    • MGC10182 antibody
    • Neural crest transcription factor Slug antibody
    • Protein sna antibody
    • Protein snail homolog 1 antibody
    • Protein snail homolog 2 antibody
    • Protein snail homolog antibody
    • Slug homolog zinc finger protein antibody
    • Slug zinc finger protein antibody
    • SLUGH antibody
    • SLUGH 1 antibody
    • SLUGH1 antibody
    • SLUGH2 antibody
    • SNA antibody
    • Sna protein antibody
    • SNAH antibody
    • SNAI 2 antibody
    • snai1 antibody
    • SNAI1_HUMAN antibody
    • Snai2 antibody
    • SNAI2_HUMAN antibody
    • Snail 2 antibody
    • Snail homolog 1 (Drosophila) antibody
    • Snail homolog 2 antibody
    • Snail2 antibody
    • WS 2D antibody
    • WS2D antibody
    • Zinc finger protein SLUG antibody
    • Zinc finger protein SNAI1 antibody
    • Zinc finger protein SNAI2 antibody
    see all

Anti-SNAIL + SLUG antibody [20C8] 图像

实验方案

Anti-SNAIL + SLUG antibody [20C8] (ab78105)参考文献

This product has been referenced in:
  • Goldman O  et al. The mesenchymal transcription factor SNAI-1 instructs human liver specification. Stem Cell Res 17:62-8 (2016). ChIP ; Human . Read more (PubMed: 27240252) »

See 1 Publication for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (DAOY, ONS76 Cells)
Loading amount 20 µg
Specification DAOY, ONS76 Cells
Gel Running Conditions Reduced Denaturing (10% SDS)
Blocking step Milk as blocking agent for 20 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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提交于 Dec 10 2009

Abcam has not validated the combination of species/application used in this Abreview.
Application ChIP
Sample Human Cell lysate - whole cell (DAOY and ONS76 Cells)
Specification DAOY and ONS76 Cells
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% Formaldehyde
Detection step Semiquantitative PCR
Positive control Whole cell lysate
Negative control Buffer Control
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提交于 Nov 09 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"