Anti-SNAIL抗体[Sn9H2] (ab31787)

概述

  • 产品名称Anti-SNAIL抗体[Sn9H2]
    参阅全部 SNAIL 一抗
  • 描述
    大鼠单克隆抗体[Sn9H2] to SNAIL
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fusion protein of Human SNAIL with 6x His® tag

  • 阳性对照
    • This antibody gave a positive signal when tested against the SNAIL recombinant protein.

性能

应用

Our Abpromise guarantee covers the use of ab31787 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 10 µg/ml. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).

靶标

  • 功能Involved in induction of the epithelial to mesenchymal transition (EMT), formation and maintenance of embryonic mesoderm, growth arrest, survival and cell migration. Binds to 3 E-boxes of the E-cadherin/CDH1 gene promoter and to the promoters of CLDN7 and KRT8 and, in association with histone demethylase KDM1A which it recruits to the promoters, causes a decrease in dimethylated H3K4 levels and represses transcription. Associates with EGR1 and SP1 to mediate tetradecanoyl phorbol acetate (TPA)-induced up-regulation of CDKN2B, possibly by binding to the CDKN2B promoter region 5'-TCACA-3. In addition, may also activate the CDKN2B promoter by itself.
  • 组织特异性Expressed in a variety of tissues with the highest expression in kidney. Expressed in mesenchymal and epithelial cell lines.
  • 序列相似性Belongs to the snail C2H2-type zinc-finger protein family.
    Contains 4 C2H2-type zinc fingers.
  • 翻译后修饰Phosphorylated by GSK3B. Once phosphorylated, it becomes a target for BTRC ubiquitination. Phosphorylation by CSNK1E, probably at Ser-104, provides the priming site for the subsequent phosphorylation by GSK3B, probably at Ser-100 and Ser-96. Phosphorylation by PAK1 may modulate its transcriptional activity by promoting increased accumulation in the nucleus. Phosphorylation at Ser-11 and Ser-92 positively regulates its functions in induction of EMT and cell survival, respectively. Phosphorylation by LATS2, upon mitotic stress, oncogenic stress or Hippo pathway activation, occurs in the nucleus and promotes nuclear retention and stabilization of total cellular protein level.
    Ubiquitinated on Lys-98, Lys-137 and Lys-146 by FBXL14 and BTRC leading to degradation. BTRC-triggered ubiquitination requires previous GSK3B-mediated SNAI1 phosphorylation. Ubiquitination induced upon interaction with NOTCH1 or TP53/p53 is mediated by MDM2.
    O-GlcNAcylation at Ser-112 is enhanced in hyperglycaemic conditions, it opposes phosphorylation by GSK3B, and stabilizes the protein.
    ADP-ribosylation by PARP1 increases protein half-life and may be involved in TGFB-induced SNAI1 up-regulation.
  • 细胞定位Nucleus. Cytoplasm. Once phosphorylated (probably on Ser-107, Ser-111, Ser-115 and Ser-119) it is exported from the nucleus to the cytoplasm where subsequent phosphorylation of the destruction motif and ubiquitination involving BTRC occurs.
  • Information by UniProt
  • 数据库链接
  • 别名
    • dJ710H13.1 antibody
    • Protein sna antibody
    • Protein snail homolog 1 antibody
    • Protein snail homolog antibody
    • SLUGH2 antibody
    • SNA antibody
    • Sna protein antibody
    • SNAH antibody
    • SNAI antibody
    • snai1 antibody
    • SNAI1_HUMAN antibody
    • Snail 1 homolog antibody
    • Snail 1 zinc finger protein antibody
    • SNAIL antibody
    • Snail homolog 1 (Drosophila) antibody
    • SNAIL, Drosophila, homolog of, 1 antibody
    • SNAIL1 antibody
    • Zinc finger protein SNAI1 antibody
    see all

Anti-SNAIL antibody [Sn9H2] 图像

  • All lanes : Anti-SNAIL antibody [Sn9H2] (ab31787) at 10 µg/ml

    Lane 1 : SNAIL (SNAI1) Human Recombinant Protein
    Lane 2 : SLUG (SNAI2) Human Recombinant Protein

    Lysates/proteins at 0.1 µg per lane.

    Secondary
    Peroxidase Conjugated AffiniPure Rabbit Anti-Rat IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 29 kDa
    Observed band size : 68 kDa (why is the actual band size different from the predicted?)


    Exposure time : 16 minutes
    Ab31787 recognizes the tagged recombinant SNAIL protein which has an expected molecular weight of 68 kDa.

Anti-SNAIL antibody [Sn9H2] (ab31787)参考文献

This product has been referenced in:
  • Weng W  et al. Metastasis-associated protein 1 promotes tumor invasion by downregulation of E-cadherin. Int J Oncol 44:812-8 (2014). Human . Read more (PubMed: 24424621) »
  • Rosivatz E  et al. Expression and nuclear localization of Snail, an E-cadherin repressor, in adenocarcinomas of the upper gastrointestinal tract. Virchows Arch 448:277-87 (2006). Read more (PubMed: 16328348) »

See all 2 Publications for this product

Product Wall

Thank you for submitting the review for the Anti-SNAIL antibody [Sn9H2] (ab31787).

I am sorry you did not find the antibody to produce any staining in your experiment. We have as yet not tested this antibody with mouse samples or in IHC-P, we ...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (embrionic mice Lung)
Specification embrionic mice Lung
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris buffer pH 9
Permeabilization No
Blocking step Gelatin 1% / goat serum 10% as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10%
Username

Mr. Raul Peña

Verified customer

提交于 Dec 03 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"