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Our Abpromise guarantee covers the use of ab53519 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 3 µg/ml.|
|IHC-P||Use at an assay dependent dilution.|
Immunohistochemical analysis of paraformaldehyde fixed mouse tissue sections, of an urethane induced lung adenocarcinoma, labelling SNAIL in the nuclei and cytoplasm. ab53519 was used at a 1/1000 dilution in 4% donkey serum for 16 hours at 4°C. Hear mediated antigen retrieval with Citrate pH6. Blocking with 4% serum for 2 hours at 25°C. Secondary antibody was a Biotinylated Anti-Goat IgG (H+L).
Western blot of Rat Kidney lysate (35µg protein in RIPA buffer) staining SNAIL with ab53519 at 0.1µg/ml. Primary incubation was 1 hour. Detected by chemiluminescence.