Anti-SMYD5抗体(ab81419)

概述

  • 产品名称Anti-SMYD5抗体
    参阅全部 SMYD5 一抗
  • 描述
    兔多克隆抗体to SMYD5
  • 经测试应用适用于: WB, ICC/IF, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Drosophila melanogaster, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within C terminal amino acids 360-409 (SRHSRHKILR ENYLFVCSCP KCLAEADEPN VTSEEEEEEE EEEEGEPEDA) of human SMYD5 (NP_006053).

  • 阳性对照
    • Hela cell lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Purified by peptide affinity chromatography method.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab81419 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 47 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC/IF Use a concentration of 5 µg/ml.
ELISA Use at an assay dependent dilution.

靶标

Anti-SMYD5 antibody 图像

  • Anti-SMYD5 antibody (ab81419) at 1 µg/ml (in 5% skim milk / PBS buffer) + Hela cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 47 kDa
    Observed band size : 42 and 47 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab81419 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab81419, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-SMYD5 antibody (ab81419)参考文献

ab81419 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 200000 cells
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (fibroblast)
Specification fibroblast
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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提交于 May 06 2014

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